Phosphorylation of the Cdc42 exchange factor Cdc24 by the PAK-like kinase Cla4 may regulate polarized growth in yeast.

Details

Serval ID
serval:BIB_17153
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Phosphorylation of the Cdc42 exchange factor Cdc24 by the PAK-like kinase Cla4 may regulate polarized growth in yeast.
Journal
Molecular Cell
Author(s)
Gulli M.P., Jaquenoud M., Shimada Y., Niederhäuser G., Wiget P., Peter M.
ISSN
1097-2765 (Print)
ISSN-L
1097-2765
Publication state
Published
Issued date
2000
Volume
6
Number
5
Pages
1155-1167
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Rho-type GTPases control many cytoskeletal rearrangements, but their regulation remains poorly understood. Here, we show that in S. cerevisiae, activation of the CDK Cdc28-Cln2 at bud emergence triggers relocalization of Cdc24, the GEF for Cdc42, from the nucleus to the polarization site, where it is stably maintained by binding to the adaptor Bem1. Locally activated Cdc42 then polarizes the cytoskeleton in a manner dependent on its effectors Bni1 and the PAK-like kinase Cla4. In addition, Cla4 induces phosphorylation of Cdc24, leading to its dissociation from Bem1 at bud tips, thereby ending polarized bud growth in vivo. Our results thus suggest a dynamic temporal and spatial regulation of the Cdc42 module: Cdc28-Cln triggers actin polarization by activating Cdc42, which in turn restricts its own activation via a negative feedback loop acting on its GEF Cdc24.
Keywords
Actins/metabolism, Adaptor Proteins, Signal Transducing, CDC28 Protein Kinase, S cerevisiae/metabolism, Carrier Proteins/metabolism, Cell Cycle Proteins/antagonists & inhibitors, Cell Cycle Proteins/chemistry, Cell Division, Cell Nucleus/metabolism, Cell Polarity, Cyclins/metabolism, Cytoplasmic Structures/chemistry, Cytoplasmic Structures/metabolism, Cytoskeleton/chemistry, Cytoskeleton/metabolism, Enzyme Activation, Feedback, Physiological, Fungal Proteins/genetics, Fungal Proteins/metabolism, G1 Phase, Guanine Nucleotide Exchange Factors, Guanosine Triphosphate/metabolism, Macromolecular Substances, Microfilament Proteins, Phosphorylation, Protein Binding, Protein Transport, Protein-Serine-Threonine Kinases/genetics, Protein-Serine-Threonine Kinases/metabolism, Proto-Oncogene Proteins/antagonists & inhibitors, Proto-Oncogene Proteins/chemistry, Recombinant Fusion Proteins/metabolism, Saccharomyces cerevisiae/cytology, Saccharomyces cerevisiae/enzymology, Saccharomyces cerevisiae Proteins, cdc42 GTP-Binding Protein/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
19/11/2007 12:10
Last modification date
20/08/2019 12:46
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