Article: article from journal or magazin.
A direct interaction of axonin-1 with NgCAM-related cell adhesion molecule (NrCAM) results in guidance, but not growth of commissural axons.
Journal of Cell Biology
An interaction of growth cone axonin-1 with the floor-plate NgCAM-related cell adhesion molecule (NrCAM) was shown to play a crucial role in commissural axon guidance across the midline of the spinal cord. We now provide evidence that axonin-1 mediates a guidance signal without promoting axon elongation. In an in vitro assay, commissural axons grew preferentially on stripes coated with a mixture of NrCAM and NgCAM. This preference was abolished in the presence of anti-axonin-1 antibodies without a decrease in neurite length. Consistent with these findings, commissural axons in vivo only fail to extend along the longitudinal axis when both NrCAM and NgCAM interactions, but not when axonin-1 and NrCAM or axonin-1 and NgCAM interactions, are perturbed. Thus, we conclude that axonin-1 is involved in guidance of commissural axons without promoting their growth.
Animals, Axons/physiology, Binding Sites, Cell Adhesion/physiology, Cell Adhesion Molecules, Cell Adhesion Molecules, Neuron-Glia/metabolism, Cell Adhesion Molecules, Neuronal/genetics, Cell Adhesion Molecules, Neuronal/metabolism, Cells, Cultured, Chick Embryo, Contactin 2, Embryonic Induction, Growth Cones/physiology, Multigene Family, Neural Pathways/embryology, Protein Binding, Recombinant Proteins/metabolism, Spinal Cord/cytology, Spinal Cord/embryology
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