Complete amino acid sequence of an integrin beta subunit (beta 7) identified in leukocytes.

Details

Serval ID
serval:BIB_154DDCBB1018
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Complete amino acid sequence of an integrin beta subunit (beta 7) identified in leukocytes.
Journal
Journal of Biological Chemistry
Author(s)
Erle D.J., Rüegg C., Sheppard D., Pytela R.
ISSN
0021-9258
Publication state
Published
Issued date
06/1991
Peer-reviewed
Oui
Volume
266
Number
17
Pages
11009-11016
Language
english
Notes
Publication types: Comparative Study ; Journal Article
Abstract
The integrins are a large family of heterodimeric cell-surface glycoproteins that play key roles in the adherence of cells to other cells and to extracellular matrix proteins. We have previously reported the identification of a novel integrin beta subunit partial cDNA from leukocytes. We have now determined the complete sequence of this subunit, designated as beta 7, from overlapping clones obtained from a PEER T leukemia cell library and a peripheral T cell library. The beta 7 cDNA contains a single large open reading frame predicted to encode a 798-amino acid protein precursor (signal peptide plus mature protein). The beta 7 protein, like the other beta subunit proteins, is predicted to contain a large extracellular portion, a transmembrane domain, and a cytoplasmic tail. The deduced beta 7 amino acid sequence is 32-46% identical to the six previously sequenced human integrin beta subunits. beta 7 is most similar to the leukocyte integrin common beta subunit (beta 2, CD18). Analysis of variant beta 7 cDNA clones and reverse transcription-polymerase chain reaction products suggest that alternatively spliced beta 7 mRNAs can be generated by the removal of exons that encode most of the cysteine-rich region of the extracellular portion of beta 7. By Northern blot analysis, beta 7 mRNA was detected in T and B cell lines and in macrophage-like cell lines, but not in any of the nonleukocyte cell lines tested. Peripheral T cells and some lymphoma lines express little beta 7 mRNA before stimulation; but after stimulation with phorbol ester, beta 7 mRNA levels increased markedly. Integrin beta 7 is expected to play a role in adhesive interactions of leukocytes.
Keywords
Amino Acid Sequence, Base Sequence, Cell Line, Cloning, Molecular, DNA Probes, DNA, Neoplasm/genetics, DNA, Neoplasm/isolation & purification, Gene Library, Humans, Integrins/genetics, Integrins/isolation & purification, Leukemia, T-Cell, Macromolecular Substances, Molecular Sequence Data, Oligonucleotide Probes, Polymerase Chain Reaction, Restriction Mapping, Sequence Homology, Nucleic Acid, T-Lymphocytes
Pubmed
Web of science
Create date
28/01/2008 8:36
Last modification date
20/08/2019 12:44
Usage data