Control of the terminal step of intracellular membrane fusion by protein phosphatase 1.

Details

Serval ID
serval:BIB_14736F40FB0C
Type
Article: article from journal or magazin.
Collection
Publications
Title
Control of the terminal step of intracellular membrane fusion by protein phosphatase 1.
Journal
Science
Author(s)
Peters C., Andrews P.D., Stark M.J., Cesaro-Tadic S., Glatz A., Podtelejnikov A., Mann M., Mayer A.
ISSN
0036-8075 (Print)
ISSN-L
0036-8075
Publication state
Published
Issued date
1999
Peer-reviewed
Oui
Volume
285
Number
5430
Pages
1084-1087
Language
english
Abstract
Intracellular membrane fusion is crucial for the biogenesis and maintenance of cellular compartments, for vesicular traffic between them, and for exo- and endocytosis. Parts of the molecular machinery underlying this process have been identified, but most of these components operate in mutual recognition of the membranes. Here it is shown that protein phosphatase 1 (PP1) is essential for bilayer mixing, the last step of membrane fusion. PP1 was also identified in a complex that contained calmodulin, the second known factor implicated in the regulation of bilayer mixing. The PP1-calmodulin complex was required at multiple sites of intracellular trafficking; hence, PP1 may be a general factor controlling membrane bilayer mixing.
Keywords
Biological Transport, Calcium/metabolism, Calmodulin/analysis, Calmodulin/metabolism, Carboxypeptidases/metabolism, Cathepsin A, Cell Membrane/metabolism, Endocytosis, Endoplasmic Reticulum/metabolism, Enzyme Inhibitors/pharmacology, Fluorescent Dyes, Golgi Apparatus/metabolism, Intracellular Membranes/metabolism, Membrane Fusion, Microcystins, Mutation, Peptides, Cyclic/pharmacology, Phosphoprotein Phosphatases/antagonists & inhibitors, Phosphoprotein Phosphatases/genetics, Protein Phosphatase 1, Pyridinium Compounds, Quaternary Ammonium Compounds, Saccharomyces cerevisiae/genetics, Saccharomyces cerevisiae/metabolism, Temperature, Vacuoles/metabolism
Pubmed
Web of science
Create date
24/01/2008 15:06
Last modification date
20/08/2019 12:43
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