A novel cyanide-inducible gene cluster helps protect Pseudomonas aeruginosa from cyanide

Details

Serval ID
serval:BIB_12DBAEBBDED1
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
A novel cyanide-inducible gene cluster helps protect Pseudomonas aeruginosa from cyanide
Journal
Environmental Microbiology Reports
Author(s)
Frangipani E., Perez-Martinez I., Williams H.D., Cherbuin G., Haas D.
ISSN
1758-2229
Publication state
Published
Issued date
2014
Volume
6
Number
1
Pages
28-34
Language
english
Abstract
Pseudomonas aeruginosa produces the toxic secondary metabolite hydrogen cyanide (HCN) at high cell population densities and low aeration. Here, we investigated the impact of HCN as a signal in cell-cell communication by comparing the transcriptome of the wild-type strain PAO1 to that of an HCN-negative mutant under cyanogenic conditions. HCN repressed four genes and induced 12 genes. While the individual functions of these genes are unknown, with one exception (i.e. a ferredoxin-dependent reductase), a highly inducible six-gene cluster (PA4129-PA4134) was found to be crucial for protection of P.aeruginosa from external HCN intoxication. A double mutant deleted for PA4129-PA4134 and cioAB (encoding cyanide-insensitive oxidase) did not grow with 100M KCN, whereas the corresponding single mutants were essentially unaffected, suggesting a synergistic action of the PA4129-PA4134 gene products and cyanide-insensitive oxidase.
Web of science
Create date
13/02/2014 10:04
Last modification date
20/08/2019 12:41
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