Article: article from journal or magazin.
Real-time PCR for type-specific identification of herpes simplex in clinical samples: evaluation of type-specific results in the context of CNS diseases.
Journal of Clinical Virology
BACKGROUND: HSV-1 and HSV-2 cause CNS infections of dissimilar clinico-pathological characteristics with prognostic and therapeutic implications. OBJECTIVES: To validate a type-specific real-time PCR that uses MGB/LNA Taqman probes and to review the virologico-clinical data of 25 eligible patients with non-neonatal CNS infections. RESULTS: This real-time PCR was evaluated against conventional PCR (26 CSF and 20 quality controls), and LightCycler assay (51 mucocutaneous, 8 CSF and 32 quality controls) and culture/immunofluorescence (75 mucocutaneous) to assess typing with independent methods. Taqman real-time PCR detected 240 HSV genomes per ml CSF, a level appropriate for the management of patients, and provided unambiguous typing for the 104 positive (62 HSV-1 and 42 HSV-2) out the 160 independent clinical samples tested. HSV type diagnosed by Taqman real-time PCR predicted final diagnosis (meningitis versus encephalitis/meningoencephalitis, p<0.001) in 24/25 patients at time of presentation, in contrast to clinical evaluation. CONCLUSIONS: Our real-time PCR, as a sensitive and specific means for type-specific HSV diagnosis, provided rapid prognostic information for patient management.
Base Sequence, DNA Probes, Encephalitis, Viral/diagnosis, Encephalitis, Viral/virology, Herpes Simplex/diagnosis, Herpes Simplex/virology, Herpesvirus 1, Human/classification, Herpesvirus 1, Human/genetics, Herpesvirus 2, Human/classification, Herpesvirus 2, Human/genetics, Humans, Meningitis, Viral/diagnosis, Meningitis, Viral/virology, Molecular Sequence Data, Polymerase Chain Reaction/methods, Species Specificity, Viral Envelope Proteins/chemistry, Viral Envelope Proteins/genetics
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