Microallelotyping defines the monoclonal or the polyclonal origin of mixed and collision endocrine-exocrine tumors of the gut.

Détails

ID Serval
serval:BIB_0F246450E233
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Microallelotyping defines the monoclonal or the polyclonal origin of mixed and collision endocrine-exocrine tumors of the gut.
Périodique
Laboratory Investigation; A Journal of Technical Methods and Pathology
Auteur(s)
Furlan D., Cerutti R., Genasetti A., Pelosi G., Uccella S., La Rosa S., Capella C.
ISSN
0023-6837 (Print)
ISSN-L
0023-6837
Statut éditorial
Publié
Date de publication
2003
Peer-reviewed
Oui
Volume
83
Numéro
7
Pages
963-971
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Résumé
Mixed endocrine-exocrine tumors of the gut are a heterogeneous group of neoplasms with uncertain histogenesis showing different morphologic and clinical features. The aim of this work is to clarify the histogenesis of these tumors by studying the genetic profile of both the endocrine and exocrine components. We performed an allelotyping analysis of five mixed endocrine-exocrine tumors (two gastric and three colonic) and one rectal collision tumor, using 35 polymorphic microsatellite markers covering a total of six chromosomes, including 3, 5q, 6, 11, 17, and 18. The loss of heterozygosity (LOH) analysis showed concurrent losses of the same allele in both the endocrine and exocrine components in all of the five mixed tumors composed by a poorly differentiated endocrine carcinoma or a well differentiated endocrine carcinoma associated with adenocarcinoma or adenoma. Among these tumors an identical LOH pattern was frequently found on chromosomes 17p, 18q, and 5q. Additional allelic losses restricted to the poorly differentiated endocrine carcinoma were often observed. On the contrary, in the only collision tumor composed by a well differentiated endocrine carcinoma associated with adenocarcinoma, completely different allelotypes between the two components were detected. These findings confirm a close genetic relationship between the two distinct histologic components within mixed endocrine-exocrine tumors, supporting the hypothesis that a monoclonal mechanism of tumorigenesis is the most frequent genetic event in mixed exocrine-endocrine tumors. The clonal divergence observed in the only collision tumor, composed by a well differentiated endocrine carcinoma associated with an adenocarcinoma, confirms the existence of double tumors growing next to each other coincidentally but showing different histogenesis and different tumorigenetic pathways.
Mots-clé
Adenocarcinoma/chemistry, Adenocarcinoma/genetics, Adult, Aged, Biomarkers, Tumor/analysis, Carcinoma, Neuroendocrine/chemistry, Carcinoma, Neuroendocrine/genetics, Clone Cells, DNA, Neoplasm/analysis, Dissection, Enteroendocrine Cells/pathology, Female, Gastrointestinal Neoplasms/chemistry, Gastrointestinal Neoplasms/genetics, Humans, Immunoenzyme Techniques, Loss of Heterozygosity, Male, Micromanipulation, Microsatellite Repeats, Middle Aged, Multiple Endocrine Neoplasia/chemistry, Multiple Endocrine Neoplasia/genetics, Polymerase Chain Reaction
Pubmed
Web of science
Création de la notice
07/09/2016 8:04
Dernière modification de la notice
20/08/2019 12:35
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