Microallelotyping defines the monoclonal or the polyclonal origin of mixed and collision endocrine-exocrine tumors of the gut.

Details

Serval ID
serval:BIB_0F246450E233
Type
Article: article from journal or magazin.
Collection
Publications
Title
Microallelotyping defines the monoclonal or the polyclonal origin of mixed and collision endocrine-exocrine tumors of the gut.
Journal
Laboratory Investigation; A Journal of Technical Methods and Pathology
Author(s)
Furlan D., Cerutti R., Genasetti A., Pelosi G., Uccella S., La Rosa S., Capella C.
ISSN
0023-6837 (Print)
ISSN-L
0023-6837
Publication state
Published
Issued date
2003
Peer-reviewed
Oui
Volume
83
Number
7
Pages
963-971
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Abstract
Mixed endocrine-exocrine tumors of the gut are a heterogeneous group of neoplasms with uncertain histogenesis showing different morphologic and clinical features. The aim of this work is to clarify the histogenesis of these tumors by studying the genetic profile of both the endocrine and exocrine components. We performed an allelotyping analysis of five mixed endocrine-exocrine tumors (two gastric and three colonic) and one rectal collision tumor, using 35 polymorphic microsatellite markers covering a total of six chromosomes, including 3, 5q, 6, 11, 17, and 18. The loss of heterozygosity (LOH) analysis showed concurrent losses of the same allele in both the endocrine and exocrine components in all of the five mixed tumors composed by a poorly differentiated endocrine carcinoma or a well differentiated endocrine carcinoma associated with adenocarcinoma or adenoma. Among these tumors an identical LOH pattern was frequently found on chromosomes 17p, 18q, and 5q. Additional allelic losses restricted to the poorly differentiated endocrine carcinoma were often observed. On the contrary, in the only collision tumor composed by a well differentiated endocrine carcinoma associated with adenocarcinoma, completely different allelotypes between the two components were detected. These findings confirm a close genetic relationship between the two distinct histologic components within mixed endocrine-exocrine tumors, supporting the hypothesis that a monoclonal mechanism of tumorigenesis is the most frequent genetic event in mixed exocrine-endocrine tumors. The clonal divergence observed in the only collision tumor, composed by a well differentiated endocrine carcinoma associated with an adenocarcinoma, confirms the existence of double tumors growing next to each other coincidentally but showing different histogenesis and different tumorigenetic pathways.
Keywords
Adenocarcinoma/chemistry, Adenocarcinoma/genetics, Adult, Aged, Biomarkers, Tumor/analysis, Carcinoma, Neuroendocrine/chemistry, Carcinoma, Neuroendocrine/genetics, Clone Cells, DNA, Neoplasm/analysis, Dissection, Enteroendocrine Cells/pathology, Female, Gastrointestinal Neoplasms/chemistry, Gastrointestinal Neoplasms/genetics, Humans, Immunoenzyme Techniques, Loss of Heterozygosity, Male, Micromanipulation, Microsatellite Repeats, Middle Aged, Multiple Endocrine Neoplasia/chemistry, Multiple Endocrine Neoplasia/genetics, Polymerase Chain Reaction
Pubmed
Web of science
Create date
07/09/2016 8:04
Last modification date
20/08/2019 12:35
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