Development and characterization of 79 nuclear markers amplifying in viviparous and oviparous clades of the European common lizard.

Details

Serval ID
serval:BIB_0EF75A0FC693
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Development and characterization of 79 nuclear markers amplifying in viviparous and oviparous clades of the European common lizard.
Journal
Genetica
Author(s)
Horreo J.L., Peláez M.L., Suárez T., Fitze P.S.
ISSN
1573-6857 (Electronic)
ISSN-L
0016-6707
Publication state
Published
Issued date
02/2018
Peer-reviewed
Oui
Volume
146
Number
1
Pages
115-121
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
The European common lizard (Zootoca vivipara) is a widely distributed species across Europe and Asia exhibiting two reproductive modes (oviparity/viviparity), six major lineages and several sublineages. It has been used to tackle a large variety of research questions, nevertheless, few nuclear DNA sequence markers have been developed for this species. Here we developed 79 new nuclear DNA sequence markers using a clonation protocol. These markers were amplified in several oviparous and viviparous specimens including samples of all extant clades, to test the amplification success and their diversity. 49.4% of the markers were polymorphic and of those, 51.3% amplified in all and 94.9% amplified in 5-7 of the extant Z. vivipara clades. These new markers will be very useful for the study of the population structure, population dynamics, and micro/macro evolution of Z. vivipara. Cross-species amplification in four lizard species (Psammodromus edwardsianus, Podarcis muralis, Lacerta bilineata, and Takydromus sexlineatus) was positive in several of the markers, and six makers amplified in all five species. The large genetic distance between P. edwardsianus and Z. vivipara further suggests that these markers may as well be employed in many other species.
Keywords
Animals, Cell Nucleus/genetics, Genetic Markers, Lizards/genetics, Oviparity, Polymorphism, Genetic, Viviparity, Nonmammalian, Clonation, Lacertidae, Nuclear DNA, Representative genes, Reptile, Zootoca vivipara
Pubmed
Web of science
Create date
01/02/2018 7:42
Last modification date
20/08/2019 12:35
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