Development and characterization of 79 nuclear markers amplifying in viviparous and oviparous clades of the European common lizard.

Détails

ID Serval
serval:BIB_0EF75A0FC693
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Development and characterization of 79 nuclear markers amplifying in viviparous and oviparous clades of the European common lizard.
Périodique
Genetica
Auteur(s)
Horreo J.L., Peláez M.L., Suárez T., Fitze P.S.
ISSN
1573-6857 (Electronic)
ISSN-L
0016-6707
Statut éditorial
Publié
Date de publication
02/2018
Peer-reviewed
Oui
Volume
146
Numéro
1
Pages
115-121
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
The European common lizard (Zootoca vivipara) is a widely distributed species across Europe and Asia exhibiting two reproductive modes (oviparity/viviparity), six major lineages and several sublineages. It has been used to tackle a large variety of research questions, nevertheless, few nuclear DNA sequence markers have been developed for this species. Here we developed 79 new nuclear DNA sequence markers using a clonation protocol. These markers were amplified in several oviparous and viviparous specimens including samples of all extant clades, to test the amplification success and their diversity. 49.4% of the markers were polymorphic and of those, 51.3% amplified in all and 94.9% amplified in 5-7 of the extant Z. vivipara clades. These new markers will be very useful for the study of the population structure, population dynamics, and micro/macro evolution of Z. vivipara. Cross-species amplification in four lizard species (Psammodromus edwardsianus, Podarcis muralis, Lacerta bilineata, and Takydromus sexlineatus) was positive in several of the markers, and six makers amplified in all five species. The large genetic distance between P. edwardsianus and Z. vivipara further suggests that these markers may as well be employed in many other species.
Mots-clé
Animals, Cell Nucleus/genetics, Genetic Markers, Lizards/genetics, Oviparity, Polymorphism, Genetic, Viviparity, Nonmammalian, Clonation, Lacertidae, Nuclear DNA, Representative genes, Reptile, Zootoca vivipara
Pubmed
Web of science
Création de la notice
01/02/2018 8:42
Dernière modification de la notice
20/08/2019 13:35
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