Cytosine methylation in CTF and Sp1 recognition sites of an HSV tk promoter: effects on transcription in vivo and on factor binding in vitro.
Details
Serval ID
serval:BIB_0E6790D23747
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Cytosine methylation in CTF and Sp1 recognition sites of an HSV tk promoter: effects on transcription in vivo and on factor binding in vitro.
Journal
Nucleic Acids Research
ISSN
0305-1048[print], 0305-1048[linking]
Publication state
Published
Issued date
1989
Volume
17
Number
24
Pages
10179-10190
Language
english
Abstract
We methylated specific cytosine residues within or immediately around the CTF and Sp1 binding sites of the Herpes simplex virus thymidine kinase promoter. The efficiency of transcription in vivo was reduced at least 50-fold compared with transcription from the unmethylated promoter. However, methylation within the CTF recognition site had no effect on the affinity of CTF for this site in vitro. Methylation of the Sp1 site resulted in only a small decrease in the affinity of this factor for its recognition site. In vivo studies showed that the same gene inserted in different vector DNAs was regulated differently by methylation in the promoter. These results show that cytosine methylation can inhibit transcription by a mechanism other than directly blocking the binding of transcription factors.
Keywords
Animals, Base Sequence, Binding Sites, Binding, Competitive, Cytosine/metabolism, DNA, Viral/genetics, DNA, Viral/metabolism, DNA-Binding Proteins/metabolism, Hela Cells, Humans, Methylation, Molecular Sequence Data, Oocytes, Promoter Regions, Genetic, Simplexvirus/enzymology, Simplexvirus/genetics, Sp1 Transcription Factor, Thymidine Kinase/genetics, Transcription Factors/metabolism, Transcription, Genetic, Xenopus laevis
Pubmed
Create date
14/10/2010 10:51
Last modification date
20/08/2019 13:35
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