The Dictyostelium discoideum EB4 gene product and a truncated mutant form of the protein are localized in prespore vesicles but absent from mature spores.
Details
Serval ID
serval:BIB_0E42BA0B8B65
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The Dictyostelium discoideum EB4 gene product and a truncated mutant form of the protein are localized in prespore vesicles but absent from mature spores.
Journal
Developmental Biology
ISSN
0012-1606 (Print)
ISSN-L
0012-1606
Publication state
Published
Issued date
1991
Volume
144
Number
1
Pages
212-214
Language
english
Abstract
Using polyclonal antibodies directed against two different parts of the predicted Dictyostelium prespore protein EB4-PSV, we have investigated the regulation and localization of its 58-kDa gene product in wild-type cells and a 31-kDa truncated form in the gene disruption transformant A3. In both strains, the protein is synthesized during aggregation and is found in the membrane fraction of prespore vesicles (PSV). In contrast to other PSV proteins, the EB4 gene product is not found in mature spores.
Keywords
Amino Acid Sequence, Dictyostelium/cytology, Dictyostelium/genetics, Fungal Proteins/genetics, Fungal Proteins/immunology, Immunoblotting, Molecular Sequence Data, Protozoan Proteins, Spores, Fungal/metabolism, Structure-Activity Relationship
Pubmed
Web of science
Create date
18/10/2012 14:13
Last modification date
20/08/2019 13:35
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