A unified design space of synthetic stripe-forming networks.

Détails

Ressource 1Télécharger: Pub_NatCommun2014.pdf (1034.72 [Ko])
Etat: Serval
Version: Final published version
ID Serval
serval:BIB_0DBB42DB9440
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
A unified design space of synthetic stripe-forming networks.
Périodique
Nature Communications
Auteur(s)
Schaerli Y., Munteanu A., Gili M., Cotterell J., Sharpe J., Isalan M.
ISSN
2041-1723 (Electronic)
ISSN-L
2041-1723
Statut éditorial
Publié
Date de publication
2014
Volume
5
Pages
4905
Langue
anglais
Résumé
Synthetic biology is a promising tool to study the function and properties of gene regulatory networks. Gene circuits with predefined behaviours have been successfully built and modelled, but largely on a case-by-case basis. Here we go beyond individual networks and explore both computationally and synthetically the design space of possible dynamical mechanisms for 3-node stripe-forming networks. First, we computationally test every possible 3-node network for stripe formation in a morphogen gradient. We discover four different dynamical mechanisms to form a stripe and identify the minimal network of each group. Next, with the help of newly established engineering criteria we build these four networks synthetically and show that they indeed operate with four fundamentally distinct mechanisms. Finally, this close match between theory and experiment allows us to infer and subsequently build a 2-node network that represents the archetype of the explored design space.

Mots-clé
Base Sequence, Cloning, Molecular, Escherichia coli, Fluorescence, Gene Regulatory Networks/genetics, Genetic Engineering/methods, Models, Genetic, Molecular Sequence Data, Plasmids/genetics, Reverse Transcriptase Polymerase Chain Reaction, Sequence Analysis, DNA, Synthetic Biology/methods
Pubmed
Web of science
Open Access
Oui
Création de la notice
01/02/2017 22:27
Dernière modification de la notice
08/05/2019 14:23
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