Independent regulation of chitin synthase and chitinase activity in Candida albicans and Saccharomyces cerevisiae

Details

Serval ID
serval:BIB_0BE9BEBF488F
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Independent regulation of chitin synthase and chitinase activity in Candida albicans and Saccharomyces cerevisiae
Journal
Microbiology
Author(s)
Selvaggini  S., Munro  C. A., Paschoud  S., Sanglard  D., Gow  N. A.
ISSN
1350-0872 (Print)
Publication state
Published
Issued date
04/2004
Volume
150
Number
Pt 4
Pages
921-8
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Apr
Abstract
Chitin is an essential structural polysaccharide in fungi that is required for cell shape and morphogenesis. One model for wall synthesis at the growing cell surface suggests that the compliance that is necessary for turgor-driven expansion of the cell wall involves a delicate balance of wall synthesis and lysis. Accordingly, de novo chitin synthesis may involve coordinated regulation of members of the CHS chitin synthase and CHT chitinase gene families. To test this hypothesis, the chitin synthase and chitinase activities of cell-free extracts were measured, as well as the chitin content of cell walls isolated from isogenic mutant strains that contained single or multiple knock-outs in members of these two gene families, in both Candida albicans and Saccharomyces cerevisiae. However, deletion of chitinase genes did not markedly affect specific chitin synthase activity, and deletion of single CHS genes had little effect on in vitro specific chitinase activity in either fungus. Chitin synthesis and chitinase production was, however, regulated in C. albicans during yeast-hypha morphogenesis. In C. albicans, the total specific activities of both chitin synthase and chitinase were higher in the hyphal form, which was attributable mainly to the activities of Chs2 and Cht3, respectively. It appeared, therefore, that chitin synthesis and hydrolysis were not coupled, but that both were regulated during yeast-hypha morphogenesis in C. albicans.
Keywords
Benzenesulfonates/pharmacology Candida albicans/drug effects/*enzymology/genetics Cell Wall/metabolism Chitin/metabolism Chitin Synthase/genetics/*metabolism Chitinase/genetics/*metabolism Fluorescent Dyes/pharmacology Gene Deletion *Gene Expression Regulation, Fungal Saccharomyces cerevisiae/drug effects/*enzymology/genetics
Pubmed
Web of science
Open Access
Yes
Create date
25/01/2008 14:40
Last modification date
20/08/2019 12:33
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