Localization of inhibins and activins in normal endocrine cells and endocrine tumors of the gut and pancreas: an immunohistochemical and in situ hybridization study.

Détails

ID Serval
serval:BIB_0B9F908612E4
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Localization of inhibins and activins in normal endocrine cells and endocrine tumors of the gut and pancreas: an immunohistochemical and in situ hybridization study.
Périodique
Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
Auteur(s)
La Rosa S., Uccella S., Marchet S., Capella C., Lloyd R.V.
ISSN
0022-1554 (Print)
ISSN-L
0022-1554
Statut éditorial
Publié
Date de publication
2004
Peer-reviewed
Oui
Volume
52
Numéro
2
Pages
217-225
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Résumé
Activins and inhibins, which belong to the TGF beta family, are composed of different combinations of alpha-, betaA-, and betaB-subunits, resulting in inhibin A (alphabetaA), inhibin B (alphabetaB), activin A (betaAbetaA), activin B (betaBbetaB), and activin AB (betaAbetaB). They regulate several cell functions, acting as paracrine/autocrine factors. Their actions, which depend on binding to specific receptors, are also modulated by follistatin. Gastroenteropancreatic (GEP) endocrine cells and endocrine tumors (ETs) produce several growth factors, but it is not well known whether they express follistatin and the various inhibin/activin subunits. We studied their expression in 65 GEP ETs using immunohistochemistry (IHC) and in situ hybridization (ISH). The alpha-subunit and follistatin were not identified in normal GEP endocrine cells and were poorly expressed in ETs. A betaA-subunit immunoreactivity (IR) was detected in A-, G-, EC-, and GIP-cells, while betaB-chain IR was present only in D-cells. The mRNAs encoding for these molecules were poorly expressed in normal tissues. BetaA- and betaB-subunits were identified in several ETs by both IHC and ISH: betaA-subunit mainly in G-cell and A-cell ETs, and betaB-subunit in D-cell, A-cell, and EC-cell ETs. Our results demonstrate a differential expression of activin/inhibin subunits among different types of GEP endocrine cells and related tumors, suggesting a role in modulation of biological functions of these normal and neoplastic endocrine cells.
Mots-clé
Activins/metabolism, Endocrine Gland Neoplasms/metabolism, Endocrine Glands/cytology, Endocrine Glands/metabolism, Enteroendocrine Cells/metabolism, Follistatin/metabolism, Gastrointestinal Neoplasms/metabolism, Humans, Immunohistochemistry, In Situ Hybridization, Inhibins/metabolism, Pancreatic Neoplasms/metabolism, Protein Subunits/metabolism
Pubmed
Web of science
Création de la notice
07/09/2016 8:04
Dernière modification de la notice
20/08/2019 12:33
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