Targeted expression of catalase to mitochondria prevents age-associated reductions in mitochondrial function and insulin resistance.

Details

Serval ID
serval:BIB_0AD8CF7C3E94
Type
Article: article from journal or magazin.
Collection
Publications
Title
Targeted expression of catalase to mitochondria prevents age-associated reductions in mitochondrial function and insulin resistance.
Journal
Cell Metabolism
Author(s)
Lee H.Y., Choi C.S., Birkenfeld A.L., Alves T.C., Jornayvaz F.R., Jurczak M.J., Zhang D., Woo D.K., Shadel G.S., Ladiges W., Rabinovitch P.S., Santos J.H., Petersen K.F., Samuel V.T., Shulman G.I.
ISSN
1932-7420 (Electronic)
ISSN-L
1550-4131
Publication state
Published
Issued date
2010
Volume
12
Number
6
Pages
668-674
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, U.S. Gov't, P.H.S.Publication Status: ppublish
Abstract
Aging-associated muscle insulin resistance has been hypothesized to be due to decreased mitochondrial function, secondary to cumulative free radical damage, leading to increased intramyocellular lipid content. To directly test this hypothesis, we examined both in vivo and in vitro mitochondrial function, intramyocellular lipid content, and insulin action in lean healthy mice with targeted overexpression of the human catalase gene to mitochondria (MCAT mice). Here, we show that MCAT mice are protected from age-induced decrease in muscle mitochondrial function (∼30%), energy metabolism (∼7%), and lipid-induced muscle insulin resistance. This protection from age-induced reduction in mitochondrial function was associated with reduced mitochondrial oxidative damage, preserved mitochondrial respiration and muscle ATP synthesis, and AMP-activated protein kinase-induced mitochondrial biogenesis. Taken together, these data suggest that the preserved mitochondrial function maintained by reducing mitochondrial oxidative damage may prevent age-associated whole-body energy imbalance and muscle insulin resistance.
Keywords
Adenosine Triphosphate/biosynthesis, Aging/metabolism, Animals, Catalase/genetics, Catalase/metabolism, DNA Damage, Energy Metabolism/physiology, Humans, Insulin/metabolism, Insulin Resistance/physiology, Male, Mice, Mice, Transgenic, Microscopy, Electron, Transmission, Mitochondria/metabolism, Mitochondria/physiology, Muscle, Skeletal/metabolism, Oxidative Stress/physiology, Oxygen Consumption/physiology, Reactive Oxygen Species/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
03/11/2015 12:32
Last modification date
20/08/2019 12:32
Usage data