Article: article from journal or magazin.
Integration of a membrane-based desalting step in a microfabricated disposable polymer injector for mass spectrometric protein analysis.
Publication types: Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
A simple desalting procedure for the coupling of a polymer microchip injector to mass spectrometry is proposed. The overall process is based on the adsorption of proteins on a poly(vinylidene difluoride) (PVDF) membrane, which are then directly eluted in the spraying solution. This microchip-based approach has been successfully applied to small drugs, peptides and proteins originally diluted in phosphate-buffered saline (PBS). Moreover, when eluting the retained proteins in small volumes, a preconcentration is obtained. The combination of single-use, mass-produceable, low-sample-consumption, easy-to-automate, miniaturized polymer injectors with easy-to-handle solution-exchange membranes makes this system particularly amenable to screening applications.
Angiotensin II/isolation & purification, Disposable Equipment, Humans, Insulin/isolation & purification, Mass Spectrometry/instrumentation, Mass Spectrometry/methods, Membranes, Artificial, Miniaturization, Myoglobin/isolation & purification, Nanotechnology, Propranolol/analysis
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