Immunocytochemical localization of oestradiol and progesterone receptors in human endometrium: a tool to assess endometrial maturation

Details

Serval ID
serval:BIB_0A2AFCFDF295
Type
Article: article from journal or magazin.
Collection
Publications
Title
Immunocytochemical localization of oestradiol and progesterone receptors in human endometrium: a tool to assess endometrial maturation
Journal
Bailliere's Clinical Obstetrics and Gynaecology
Author(s)
Bouchard  P., Marraoui  J., Massai  M. R., Medalie  D. A., De Ziegler  D., Perrot-Applanat  M., Frydman  R., Bergeron  C.
ISSN
0950-3552
Publication state
Published
Issued date
03/1991
Peer-reviewed
Oui
Volume
5
Number
1
Pages
107-15
Notes
Journal Article --- Old month value: Mar
Abstract
Uterine oestrogen (ER) and progesterone (PR) receptors are subject to fine hormonal control by oestradiol and progesterone. In order to assess the role of ER and PR measurement in the evaluation of endometrial maturation, both receptors were studied by immunocytochemical techniques using monoclonal antibodies during the menstrual cycle, and in women with inactive ovaries treated by different regimens of hormonal substitution with oestradiol and progesterone. During the normal menstrual cycle, the concentrations and distribution of ER and PR changed markedly. During the mid follicular period (days 7-8), a small proportion of stromal and glandular cells stained positively for PR while staining for ER was more intense and more frequent. During the late follicular phase and early luteal period (days 9-19), the staining for PR increased markedly in glandular cells. During the mid and late luteal phase (days 21-27), ER and PR staining disappeared in glandular cells. Thus, while oestradiol increases the staining for ER and PR in both glands and stroma, progesterone decreases ER and PR staining in the glands in a dramatic fashion. These variations, especially the disappearance of PR under the effect of progesterone, are potentially useful for studying the cumulative effect of progesterone on endometrial maturation. This was confirmed in anovulatory women, where a late luteal phase aspect was observed, i.e. the absence of a reduction in ER and PR in glandular cells. In women with ovarian failure, the disappearance of ER and PR in glandular cells is correlated with the duration of progesterone therapy.(ABSTRACT TRUNCATED AT 250 WORDS)
Keywords
Adolescent Adult Antibodies, Monoclonal Arteries/chemistry Endometrium/*chemistry/physiology Female Humans Immunoenzyme Techniques Luteal Phase/physiology Menstrual Cycle/metabolism Ovarian Diseases/drug therapy/metabolism Receptors, Estradiol/*analysis Receptors, Progesterone/*analysis Uterus/blood supply
Pubmed
Web of science
Create date
28/02/2008 11:37
Last modification date
20/08/2019 12:32
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