R factor variants with enhanced sex factor activity in Pseudomonas aeruginosa.

Détails

ID Serval
serval:BIB_097AF864BFB2
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
R factor variants with enhanced sex factor activity in Pseudomonas aeruginosa.
Périodique
Molecular and General Genetics
Auteur(s)
Haas D., Holloway B.W.
ISSN
0026-8925 (Print)
ISSN-L
0026-8925
Statut éditorial
Publié
Date de publication
1976
Volume
144
Numéro
3
Pages
243-251
Langue
anglais
Résumé
The R factor R68 readily promotes chromosome transfer in Pseudomonas aeruginosa strain PAT, but shows little such sex factor activity in strain PAO. A variant of this plasmid, R68.45, has been isolated which produces recombinants in PAO plate matings at frequencies of 10(-3)--10(-5) per donor cell for markers in the 0-60 min region of the chromosome. Little or nor chromosome transfer was shown in liquid media. The kinetics of chromosome transfer were studied by interrupting matings on solid media with nalidixic acid. Five chromosomal markers, mapping in widely spaced regions of the chromosome all entered 3-5 min after initiation of mating. These results, combined with linkage studies, indicated that R68.45, unlike the Pseudomonas sex factors FP2 and FP39, promotes chromosome transfer from a range of origin sites and can thus be used for mapping the region of the P. aeruginosa chromosome later than 40 min. R68.45 and other similar variants were isolated from rare chromosomal recombinants appearing in crosses between PAO(R68) donors and PAO recipients in which selection for ARGB+ was made. Selection for other chromosomal markers did not result in such variants suggesting that plasmides of the R68.45 type arise by recombination of genetic material between the R68 plasmid and certain regions of the bacterial chromosome.
Mots-clé
Chromosome Mapping, Crosses, Genetic, Drug Resistance, Microbial, Genetic Variation, Nalidixic Acid/pharmacology, Pseudomonas aeruginosa/drug effects, R Factors, Recombination, Genetic, Species Specificity
Pubmed
Web of science
Création de la notice
25/01/2008 18:01
Dernière modification de la notice
03/03/2018 13:31
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