Human ATP1AL1 gene encodes a ouabain-sensitive H-K-ATPase

Détails

ID Serval
serval:BIB_0813614AD621
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Human ATP1AL1 gene encodes a ouabain-sensitive H-K-ATPase
Périodique
American Journal of Physiology
Auteur(s)
Modyanov  N. N., Mathews  P. M., Grishin  A. V., Beguin  P., Beggah  A. T., Rossier  B. C., Horisberger  J. D., Geering  K.
ISSN
0363-6143
Statut éditorial
Publié
Date de publication
10/1995
Volume
269
Numéro
4 Pt 1
Pages
C992-7
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Oct
Résumé
The cDNA for ATP1AL1, the fifth member of the human Na-K-adenosinetriphosphatase (ATPase)/H-K-ATPase gene family, was recently cloned (A. V. Grishin, V. E. Sverdlov, M. B. Kostina, and N. N. Modyanov. FEBS Lett. 349: 144-150, 1994). The encoded protein (ATP1AL1) has all the primary structural features common to the catalytic alpha-subunit of ion-transporting P-type ATPases and is similar (63-64% identity) to the Na-K-ATPase alpha-subunit isoforms and the gastric H-K-ATPase alpha-subunit. In this study, ATP1AL1 was expressed in Xenopus laevis oocytes in combination with the beta-subunit of rabbit gastric H-K-ATPase. The functional properties of the stable alpha/beta-complex were studied by 86Rb+ uptake and demonstrated that ATP1AL1 is a novel human K(+)-dependent ATPase [apparent half-constant activation/(K1/2) for K+ approximately 375 microM)]. ATP1AL1-mediated inward K+ transport was inhibited by ouabain (inhibition constant approximately 13 microM) and was found to be inhibited by high concentrations of SCH-28080 (approximately 70% at 500 microM). ATP1AL1 expression resulted in the alkalinization of the oocytes' cytoplasm and ouabain-sensitive proton extrusion, as measured with pH-sensitive microelectrodes. These data argue that ATP1AL1 is the catalytic alpha-subunit of a human nongastric P-type ATPase capable of exchanging extracellular potassium for intracellular protons.
Mots-clé
Animals Biological Transport/drug effects *Genes H(+)-K(+)-Exchanging ATPase/*genetics/metabolism Humans Imidazoles/pharmacology Oocytes/metabolism Ouabain/*pharmacokinetics Potassium/metabolism Rabbits Xenopus laevis
Pubmed
Web of science
Création de la notice
24/01/2008 13:28
Dernière modification de la notice
03/03/2018 13:29
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