Subcellular fractionation of stored red blood cells reveals a compartment-based protein carbonylation evolution.

Details

Serval ID
serval:BIB_08071938706F
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Subcellular fractionation of stored red blood cells reveals a compartment-based protein carbonylation evolution.
Journal
Journal of Proteomics
Author(s)
Delobel J., Prudent M., Rubin O., Crettaz D., Tissot J.D., Lion N.
ISSN
1876-7737 (Electronic)
Publication state
Published
Issued date
2012
Peer-reviewed
Oui
Volume
76
Number
Spec No.
Pages
181-193
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Abstract
During blood banking, erythrocytes undergo storage lesions, altering or degrading their metabolism, rheological properties, and protein content. Carbonylation is a hallmark of protein oxidative lesions, thus of red blood cell oxidative stress. In order to improve global erythrocyte protein carbonylation assessment, subcellular fractionation has been established, allowing us to work on four different protein populations, namely soluble hemoglobin, hemoglobin-depleted soluble fraction, integral membrane and cytoskeleton membrane protein fractions. Carbonylation in erythrocyte-derived microparticles has also been investigated. Carbonylated proteins were derivatized with 2,4-dinitrophenylhydrazine (2,4-DNPH) and quantified by western blot analyses. In particular, carbonylation in the cytoskeletal membrane fraction increased remarkably between day 29 and day 43 (P<0.01). Moreover, protein carbonylation within microparticles released during storage showed a two-fold increase along the storage period (P<0.01). As a result, carbonylation of cytoplasmic and membrane protein fractions differs along storage, and the present study allows explaining two distinct steps in global erythrocyte protein carbonylation evolution during blood banking. This article is part of a Special Issue entitled: Integrated omics.
Keywords
Blood Preservation, Cell-Derived Microparticles/metabolism, Erythrocytes/cytology, Erythrocytes/metabolism, Humans, Protein Carbonylation, Time Factors
Pubmed
Web of science
Create date
08/05/2013 14:07
Last modification date
20/08/2019 12:30
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