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Small ubiquitin-like modifier conjugating enzyme with active site mutation acts as dominant negative inhibitor of SUMO conjugation in arabidopsis(F).
Journal of Integrative Plant Biology
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Small ubiquitin-like modifier (SUMO) conjugation affects a broad range of processes in plants, including growth, flower initiation, pathogen defense, and responses to abiotic stress. Here, we investigate in vivo and in vitro a SUMO conjugating enzyme with a Cys to Ser change in the active site, and show that it has a dominant negative effect. In planta expression significantly perturbs normal development, leading to growth retardation, early flowering and gene expression changes. We suggest that the mutant protein can serve as a probe to investigate sumoylation, also in plants for which poor genetic infrastructure precludes analysis via loss-of-function mutants.
Arabidopsis/enzymology, Arabidopsis/metabolism, Blotting, Western, Mutation, Small Ubiquitin-Related Modifier Proteins/antagonists & inhibitors, Small Ubiquitin-Related Modifier Proteins/metabolism, Sumoylation, Transcription, Genetic, Ubiquitin/metabolism
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