Phospholemman (FXYD1) associates with Na,K-ATPase and regulates its transport properties
Details
Serval ID
serval:BIB_0702C08AC4AE
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Phospholemman (FXYD1) associates with Na,K-ATPase and regulates its transport properties
Journal
Proceedings of the National Academy of Sciences of the United States of America
ISSN
0027-8424 (Print)
Publication state
Published
Issued date
08/2002
Volume
99
Number
17
Pages
11476-81
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Aug 20
Research Support, Non-U.S. Gov't --- Old month value: Aug 20
Abstract
A family of small, single-span membrane proteins (the FXYD family) has recently been defined based on their sequence and structural homology. Some members of this family have already been identified as tissue-specific regulators of Na,K-ATPase (NKA). In the present study, we demonstrate that phospholemman (PLM) (FXYD1), so far considered to be a heart- and muscle-specific channel or channel-regulating protein, associates specifically and stably with six different alpha-beta isozymes of NKA after coexpression in Xenopus oocytes, and with alpha1-beta, and less efficiently with alpha2-beta isozymes, in native cardiac and skeletal muscles. Stoichiometric association of PLM with NKA occurs posttranslationally either in the Golgi or the plasma membrane. Interaction of PLM with NKA induces a small decrease in the external K+ affinity of alpha1-beta1 and alpha2-beta1 isozymes and a nearly 2-fold decrease in the internal Na+ affinity. In conclusion, this study demonstrates that PLM is a tissue-specific regulator of NKA that may play an essential role in muscle contractility.
Keywords
Animals
Biological Transport
Calcium-Transporting ATPases/metabolism
Cattle
Cloning, Molecular
Dogs
Endoplasmic Reticulum/enzymology
Female
Heart/physiology
Humans
Kidney/physiology
Membrane Potentials
Membrane Proteins/genetics/*metabolism
Microsomes/metabolism
Muscle, Skeletal/physiology
Na(+)-K(+)-Exchanging ATPase/*metabolism
Oocytes/physiology
Phosphoproteins/genetics/*metabolism
Protein Subunits
Rats
Recombinant Proteins/metabolism
Sarcoplasmic Reticulum/enzymology
Sarcoplasmic Reticulum Calcium-Transporting ATPases
Xenopus laevis
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 13:28
Last modification date
20/08/2019 13:29