Oscillating expression of c-Hey2 in the presomitic mesoderm suggests that the segmentation clock may use combinatorial signaling through multiple interacting bHLH factors

Details

Serval ID
serval:BIB_022BEEB261BB
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Oscillating expression of c-Hey2 in the presomitic mesoderm suggests that the segmentation clock may use combinatorial signaling through multiple interacting bHLH factors
Journal
Developmental Biology
Author(s)
Leimeister  C., Dale  K., Fischer  A., Klamt  B., Hrabe de Angelis  M., Radtke  F., McGrew  M. J., Pourquie  O., Gessler  M.
ISSN
0012-1606 (Print)
Publication state
Published
Issued date
11/2000
Volume
227
Number
1
Pages
91-103
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Nov 1
Abstract
Vertebrate somitogenesis comprises the generation of a temporal periodicity, the establishment of anteroposterior compartment identity, and the translation of the temporal periodicity into the metameric pattern of somites. Molecular players at each of these steps are beginning to be identified. Especially, members of the Notch signaling cascade appear to be involved in setting up the somitogenesis clock and subsequent events. We had previously demonstrated specific expression of the mHey1 and mHey2 basic helix-loop-helix (bHLH) factors during somitogenesis. Here we show that perturbed Notch signaling in Dll1 and Notch1 knockout mutants affects this expression in the presomitic mesoderm (PSM) and the somites. In the caudal PSM, however, mHey2 expression is maintained and thus is likely to be independent of Notch signaling. Furthermore, we analysed the dynamic expression of the respective chicken c-Hey1 and c-Hey2 genes during somitogenesis. Not only is c-Hey2 rhythmically expressed across the chicken presomitic mesoderm like c-hairy1, but its transcription is similarly independent of de novo protein synthesis. In contrast, the dynamic expression of c-Hey1 is restricted to the anterior segmental plate. Both c-Hey genes are coexpressed with c-hairy1 in the posterior somite half. Further in vitro and in vivo interaction assays demonstrated direct homo- and heterodimerisation between these hairy-related bHLH proteins, suggesting a combinatorial action in both the generation of a temporal periodicity and the anterior-posterior somite compartmentalisation.
Keywords
Amino Acid Sequence Animals *Avian Proteins Basic Helix-Loop-Helix Transcription Factors Biological Clocks/*physiology Chick Embryo *Gene Expression Regulation, Developmental *Helix-Loop-Helix Motifs In Situ Hybridization Membrane Proteins/genetics/physiology Mesoderm/*metabolism Mice Mice, Knockout Molecular Sequence Data Morphogenesis Protein Binding Proteins/metabolism RNA, Messenger/analysis/genetics Receptor, Notch1 *Receptors, Cell Surface Repressor Proteins/chemistry/genetics/metabolism Sequence Alignment *Signal Transduction Somites/metabolism Transcription Factors/chemistry/genetics/*metabolism Two-Hybrid System Techniques
Pubmed
Web of science
Open Access
Yes
Create date
28/01/2008 11:39
Last modification date
20/08/2019 12:24
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