Transcription of the human loricrin gene in vitro is induced by calcium and cell density and suppressed by retinoic acid

Détails

ID Serval
serval:BIB_010859A06D26
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Transcription of the human loricrin gene in vitro is induced by calcium and cell density and suppressed by retinoic acid
Périodique
Journal of Investigative Dermatology
Auteur(s)
Hohl  D., Lichti  U., Breitkreutz  D., Steinert  P. M., Roop  D. R.
ISSN
0022-202X (Print)
Statut éditorial
Publié
Date de publication
04/1991
Volume
96
Numéro
4
Pages
414-8
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S. --- Old month value: Apr
Résumé
We have previously shown that loricrin is a major component of the cornified cell envelope (CE) expressed late in epidermal differentiation in the granular layers of normal skin. Normal human keratinocytes were cultured under various conditions and loricrin mRNA levels were assessed at various time points. Only Ca++ concentrations above 0.1 mM Ca++ were permissive for the expression of lori-crin mRNA. Maximal mRNA levels were found at 0.35 mM Ca++ and a critical cell density appeared to be required for optimal accumulation of loricrin transcripts. Retinoic acid (RA) at 10(-7) to 10(-9) M completely blocked Ca+(+)-induced loricrin mRNA synthesis when applied simultaneously. Furthermore, addition of RA to cultures already exposed to higher Ca++ levels resulted in the complete loss of loricrin mRNA within 48-72 h. So far, no other components of the CE have been shown to be suppressed by RA. However, similar patterns of expression were reported for filaggrin, a matrix protein also expressed late in epidermal differentiation. Therefore, we compared the mRNA levels of loricrin and filaggrin and found them to change in parallel in response to the various culture conditions. These results suggest that Ca++, cell density, and RA are crucial regulators of loricrin expression in vitro and that the transcriptional control of loricrin and filaggrin expression in the epidermis are closely coordinated.
Mots-clé
Blotting, Northern Calcium/*pharmacology Cell Count/*drug effects Female Humans Intermediate Filament Proteins/genetics Membrane Proteins/*genetics RNA, Messenger/analysis Transcription, Genetic Tretinoin/*pharmacology
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/01/2008 17:36
Dernière modification de la notice
08/05/2019 13:40
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