Rescue of the albino phenotype by introduction of a functional tyrosinase gene into mice

Détails

ID Serval
serval:BIB_FFDEEE5CE5AD
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Rescue of the albino phenotype by introduction of a functional tyrosinase gene into mice
Périodique
EMBO Journal
Auteur(s)
Beermann F., Ruppert S., Hummler E., Bosch F. X., Müller G., Rüther U., Schütz G.
ISSN
0261-4189 (Print)
Statut éditorial
Publié
Date de publication
09/1990
Volume
9
Numéro
9
Pages
2819-2826
Notes
Journal Article Research Support, Non-U.S. Gov't --- Old month value: Sep
Résumé
The c-locus of the mouse is thought to encode tyrosinase, the key enzyme for melanin synthesis in melanocytes of the skin and the eye. Recently, a mouse cDNA was isolated and shown to confer tyrosine activity on a cell line which expressed no specialized functions for melanin synthesis. To verify that the isolated tyrosinase gene is encoded at the genetically well characterized c-locus, a minigene was assembled from tyrosinase cDNA and tyrosinase genomic DNA and used for generation of transgenic mice. Following microinjection of this construct into fertilized eggs of an albino mouse strain, transgenic mice were obtained which showed pigmentation in skin and eyes. By in situ hybridization, we show expression of the transgene in melanocytes of the hairbulb and in the pigmented cell layers of the eye. We conclude that we have rescued the albino mutation (c/c) by introduction and expression of a functional tyrosinase gene.
Mots-clé
Albinism/enzymology/*genetics Amino Acid Sequence Animals Base Sequence Catechol Oxidase/*genetics *Genes Hair Color/genetics Mice Mice, Inbred Strains Mice, Transgenic Molecular Sequence Data Monophenol Monooxygenase/*genetics Mutation Oligonucleotide Probes Plasmids Restriction Mapping
Pubmed
Web of science
Création de la notice
24/01/2008 12:42
Dernière modification de la notice
20/08/2019 16:30
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