Novel strategies for expansion of tooth epithelial stem cells and ameloblast generation.

Détails

Ressource 1Télécharger: 201. Martiin et al.pdf (6392.44 [Ko])
Etat: Public
Version: Final published version
Licence: CC BY 4.0
ID Serval
serval:BIB_FF7584C4D979
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Novel strategies for expansion of tooth epithelial stem cells and ameloblast generation.
Périodique
Scientific reports
Auteur(s)
Binder M., Biggs L.C., Kronenberg M.S., Schneider P., Thesleff I., Balic A.
ISSN
2045-2322 (Electronic)
ISSN-L
2045-2322
Statut éditorial
Publié
Date de publication
18/03/2020
Peer-reviewed
Oui
Volume
10
Numéro
1
Pages
4963
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Résumé
Enamel is secreted by ameloblasts derived from tooth epithelial stem cells (SCs). Humans cannot repair or regenerate enamel, due to early loss of tooth epithelial SCs. Contrarily in the mouse incisors, epithelial SCs are maintained throughout life and endlessly generate ameloblasts, and thus enamel. Here we isolated Sox2-GFP+ tooth epithelial SCs which generated highly cellular spheres following a novel in vitro strategy. This system enabled analysis of SC regulation by various signaling molecules, and supported the stimulatory and inhibitory roles of Shh and Bmp, respectively; providing better insight into the heterogeneity of the SCs. Further, we generated a novel mouse reporter, Enamelin-tdTomato for identification of ameloblasts in live tissues and cells, and used it to demonstrate presence of ameloblasts in the new 3D co-culture system of dental SCs. Collectively, our results provide means of generating 3D tooth epithelium from adult SCs which can be utilized toward future generation of enamel.
Pubmed
Open Access
Oui
Création de la notice
01/04/2020 21:23
Dernière modification de la notice
28/04/2020 7:10
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