Orosomucoid (alpha-1 acid glycoprotein) phenotyping by use of immobilized pH gradients with 8 M urea and immunoblotting. A new variant encountered in a population study.

Détails

ID Serval
serval:BIB_FF693CFD2E21
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Orosomucoid (alpha-1 acid glycoprotein) phenotyping by use of immobilized pH gradients with 8 M urea and immunoblotting. A new variant encountered in a population study.
Périodique
Human Genetics
Auteur(s)
Eap C.B., Cuendet C., Baumann P.
ISSN
0340-6717 (Print)
ISSN-L
0340-6717
Statut éditorial
Publié
Date de publication
1988
Peer-reviewed
Oui
Volume
80
Numéro
2
Pages
183-185
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Résumé
Orosomucoid (ORM) phenotyping has been performed on 329 unrelated Swiss subjects, using immobilized pH gradients with 8 M urea and 2% v/v 2-mercaptoethanol followed by immunoblotting. After desialylation the band patterns of ORM confirmed that the polymorphism of the structural locus ORM1 is controlled by three codominant autosomal alleles (ORM1*F1, ORM1*S and ORM1*F2). One rare and one new allele were detected. The rare variant, tentatively assigned to the second structural locus ORM2, is observed in a cathodal position and named ORM2 B1. The new variant, tentatively assigned to the first structural locus ORM1, is observed in a region located between ORM1 S and ORM1 F2, and named ORM1 F3. Moreover, the pI values of the ORM variants have been measured accurately with Immobiline Dry Plates (LKB): they were found to be within the pH range 4.93-5.14.
Mots-clé
Genes, Genes, Dominant, Humans, Hydrogen-Ion Concentration, Immunoblotting, Orosomucoid/genetics, Phenotype, Urea
Pubmed
Web of science
Création de la notice
01/03/2013 9:58
Dernière modification de la notice
20/08/2019 16:29
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