Virulence of alkaline protease-deficient mutants of Aspergillus fumigatus

Détails

ID Serval
serval:BIB_FF6456667F3F
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Virulence of alkaline protease-deficient mutants of Aspergillus fumigatus
Périodique
FEMS Microbiology Letters
Auteur(s)
Monod  M., Paris  S., Sarfati  J., Jaton-Ogay  K., Ave  P., Latge  J. P.
ISSN
0378-1097 (Print)
Statut éditorial
Publié
Date de publication
01/1993
Volume
106
Numéro
1
Pages
39-46
Notes
Journal Article --- Old month value: Jan 1
Résumé
The gene encoding the secreted alkaline protease, a suspected virulence factor of Aspergillus fumigatus, was inactivated by gene disruption. The disruption was performed by transformation of a pathogenic strain of the fungus with a linear DNA fragment carrying the gene from which the central part was replaced by the selectable Escherichia coli hygromycin B dominant resistance marker. Two transformants were shown to produce no alkaline protease. Restriction fragment analysis of the DNA of these two transformants was consistent for chromosomal integration of the disrupted gene by homologous recombination. Both isogenic alkaline protease-producing and non-producing A. fumigatus strains invaded lung tissues, causing comparable mortality in immunosuppressed mice. A significant residual proteolytic activity observed in alkaline protease non-producing strain cultures could play a role in the invasion of the tissues by the fungus.
Mots-clé
Animals Aspergillus fumigatus/enzymology/*pathogenicity Chromosome Mapping Cloning, Molecular Collagen/metabolism Drug Resistance, Microbial Elastin/metabolism Electrophoresis, Polyacrylamide Gel Gene Deletion Genes, Fungal/physiology Hygromycin B Lung/pathology Male Mice Mice, Inbred Strains Polymorphism, Restriction Fragment Length Protein Biosynthesis Serine Endopeptidases/*deficiency/genetics Transformation, Genetic Virulence/physiology
Pubmed
Web of science
Création de la notice
25/01/2008 17:46
Dernière modification de la notice
20/08/2019 17:29
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