Critical role of P-selectin and lymphocyte function antigen-1 in radiation-induced leukocyte-endothelial cell interactions in the colon.

Détails

ID Serval
serval:BIB_FF4FA5335ECB
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Critical role of P-selectin and lymphocyte function antigen-1 in radiation-induced leukocyte-endothelial cell interactions in the colon.
Périodique
Diseases of the Colon and Rectum
Auteur⸱e⸱s
Mihaescu A., Thornberg C., Mattsson S., Wang Y., Jeppsson B., Thorlacius H.
ISSN
0012-3706 (Print)
ISSN-L
0012-3706
Statut éditorial
Publié
Date de publication
2007
Volume
50
Numéro
12
Pages
2194-2202
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
PURPOSE: Radiation therapy is frequently used in treating different types of tumors, although associated with serious side effects, such as fibrosis and complicated diarrhea. This study was designed to define the adhesive mechanisms behind radiotherapy-induced leukocyte recruitment in the colon. METHODS: All mice, except control animals, were radiated with a single dose of 20 Gy. Mice were pretreated with an isotype-matched control antibody or a monoclonal antibody directed against P-selectin. In separate experiments, lymphocyte function antigen-1-deficient animals were used. Leukocyte rolling and firm adhesion were determined by use of inverted intravital fluorescence microscopy 16 hours after radiation. RESULTS: It was found that immunoneutralization of P-selectin reduced leukocyte rolling by 83 percent and adhesion by 87 percent in radiated mice. Moreover, radiation-induced leukocyte adhesion in LFA-1-deficient mice was decreased by 94 percent compared with wild-type animals. CONCLUSIONS: This study demonstrates that leukocyte rolling is mediated by P-selectin and that firm leukocyte adhesion is supported by lymphocyte function antigen-1 in radiation-induced enteritis. Moreover, P-selectin-dependent leukocyte rolling is a precondition for subsequent leukocyte adhesion in radiation-induced intestinal injury. Thus, targeting P-selectin and/or lymphocyte function antigen-1 may protect against pathologic inflammation in the colon induced by radiotherapy
Mots-clé
Animals, Cell Adhesion/radiation effects, Colitis/etiology, Colitis/metabolism, Colon/pathology, Colon/radiation effects, DNA, Neoplasm/genetics, Disease Models, Animal, Endothelial Cells/pathology, Gene Expression Regulation, Neoplastic, Leukocyte Count, Lymphocyte Function-Associated Antigen-1/genetics, Lymphocyte Function-Associated Antigen-1/metabolism, Male, Mice, Mice, Inbred C57BL, Microscopy, Fluorescence, P-Selectin/metabolism, Radiation Injuries, Experimental/metabolism, Radiation Injuries, Experimental/pathology, Reverse Transcriptase Polymerase Chain Reaction
Pubmed
Web of science
Création de la notice
29/01/2008 18:34
Dernière modification de la notice
20/08/2019 16:29
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