Comparison of differential gene expression to water stress among bacteria with relevant pollutant-degradation properties.

Détails

ID Serval
serval:BIB_FF2CB4936A57
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Comparison of differential gene expression to water stress among bacteria with relevant pollutant-degradation properties.
Périodique
Environmental microbiology reports
Auteur(s)
Moreno-Forero S.K., Rojas E., Beggah S., van der Meer J.R.
ISSN
1758-2229 (Electronic)
ISSN-L
1758-2229
Statut éditorial
Publié
Date de publication
02/2016
Volume
8
Numéro
1
Pages
91-102
Langue
anglais
Notes
Publication types: Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Resistance to semi-dry environments has been considered a crucial trait for superior growth and survival of strains used for bioaugmentation in contaminated soils. In order to compare water stress programmes, we analyse differential gene expression among three phylogenetically different strains capable of aromatic compound degradation: Arthrobacter chlorophenolicus A6, Sphingomonas wittichii RW1 and Pseudomonas veronii  1YdBTEX2. Standardized laboratory-induced water stress was imposed by shock exposure of liquid cultures to water potential decrease, induced either by addition of solutes (NaCl, solute stress) or by addition of polyethylene glycol (matric stress), both at absolute similar stress magnitudes and at those causing approximately similar decrease of growth rates. Genome-wide differential gene expression was recorded by micro-array hybridizations. Growth of P. veronii 1YdBTEX2 was the most sensitive to water potential decrease, followed by S. wittichii RW1 and A. chlorophenolicus A6. The number of genes differentially expressed under decreasing water potential was lowest for A. chlorophenolicus A6, increasing with increasing magnitude of the stress, followed by S. wittichii RW1 and P. veronii 1YdBTEX2. Gene inspection and gene ontology analysis under stress conditions causing similar growth rate reduction indicated that common reactions among the three strains included diminished expression of flagellar motility and increased expression of compatible solutes (which were strain-specific). Furthermore, a set of common genes with ill-defined function was found between all strains, including ABC transporters and aldehyde dehydrogenases, which may constitute a core conserved response to water stress. The data further suggest that stronger reduction of growth rate of P. veronii 1YdBTEX2 under water stress may be an indirect result of the response demanding heavy NADPH investment, rather than the presence or absence of a suitable stress defence mechanism per se.

Mots-clé
Dehydration, Gene Expression Profiling, Microarray Analysis, Micrococcaceae/genetics, Micrococcaceae/metabolism, Pseudomonas/genetics, Pseudomonas/growth & development, Pseudomonas/metabolism, Sphingomonas/genetics, Sphingomonas/metabolism, Water Pollutants/metabolism
Pubmed
Open Access
Oui
Création de la notice
04/04/2016 9:54
Dernière modification de la notice
20/08/2019 17:29
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