Cross-priming of naive CD8 T cells against melanoma antigens using dendritic cells loaded with killed allogeneic melanoma cells

Détails

Ressource 1Télécharger: BIB_FEB7ADFCEB2F.P001.pdf (328.43 [Ko])
Etat: Public
Version: de l'auteur
ID Serval
serval:BIB_FEB7ADFCEB2F
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Cross-priming of naive CD8 T cells against melanoma antigens using dendritic cells loaded with killed allogeneic melanoma cells
Périodique
Journal of Experimental Medicine
Auteur(s)
Berard  F., Blanco  P., Davoust  J., Neidhart-Berard  E. M., Nouri-Shirazi  M., Taquet  N., Rimoldi  D., Cerottini  J. C., Banchereau  J., Palucka  A. K.
ISSN
0022-1007 (Print)
Statut éditorial
Publié
Date de publication
12/2000
Volume
192
Numéro
11
Pages
1535-44
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Dec 4
Résumé
The goal of tumor immunotherapy is to elicit immune responses against autologous tumors. It would be highly desirable that such responses include multiple T cell clones against multiple tumor antigens. This could be obtained using the antigen presenting capacity of dendritic cells (DCs) and cross-priming. That is, one could load the DC with tumor lines of any human histocompatibility leukocyte antigen (HLA) type to elicit T cell responses against the autologous tumor. In this study, we show that human DCs derived from monocytes and loaded with killed melanoma cells prime naive CD45RA(+)CD27(+)CD8(+) T cells against the four shared melanoma antigens: MAGE-3, gp100, tyrosinase, and MART-1. HLA-A201(+) naive T cells primed by DCs loaded with HLA-A201(-) melanoma cells are able to kill several HLA-A201(+) melanoma targets. Cytotoxic T lymphocyte priming towards melanoma antigens is also obtained with cells from metastatic melanoma patients. This demonstration of cross-priming against shared tumor antigens builds the basis for using allogeneic tumor cell lines to deliver tumor antigens to DCs for vaccination protocols.
Mots-clé
Antigens, Neoplasm/*immunology Cancer Vaccines/immunology Cell Differentiation Cells, Cultured Dendritic Cells/*immunology Humans K562 Cells Melanoma/*immunology/pathology Membrane Glycoproteins/immunology Monophenol Monooxygenase/immunology Neoplasm Proteins/immunology Neoplasm Staging T-Lymphocytes, Cytotoxic/cytology/*immunology Tumor Cells, Cultured
Pubmed
Web of science
Open Access
Oui
Création de la notice
28/01/2008 12:13
Dernière modification de la notice
20/08/2019 17:29
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