A switch in mitotic histone H4 lysine 20 methylation status is linked to M phase defects upon loss of HCF-1.

Détails

ID Serval
serval:BIB_FEB155C2ACE6
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
A switch in mitotic histone H4 lysine 20 methylation status is linked to M phase defects upon loss of HCF-1.
Périodique
Molecular Cell
Auteur⸱e⸱s
Julien E., Herr W.
ISSN
1097-2765[print], 1097-2765[linking]
Statut éditorial
Publié
Date de publication
2004
Peer-reviewed
Oui
Volume
14
Numéro
6
Pages
713-725
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Résumé
The abundant chromatin-associated human factor HCF-1 is a heterodimeric complex of HCF-1N and HCF-1C subunits that are essential for two stages of the cell cycle. The HCF-1N subunit promotes G1 phase progression, whereas the HCF-1C subunit ensures proper cytokinesis at completion of M phase. How the HCF-1C subunit functions is unknown. Here, we show that HCF-1C subunit depletion causes extensive mitotic defects, including a switch from monomethyl to dimethyl lysine 20 of histone H4 (H4-K20) and defective chromosome alignment and segregation. Consistent with these activities, the HCF-1C subunit can associate with chromatin independently of the HCF-1N subunit and regulates the expression of the H4-K20 methyltransferase PR-Set7. Indeed, upregulation of PR-Set7 expression upon loss of HCF-1 leads to improper mitotic H4-K20 methylation and cytokinesis defects. These results establish the HCF-1C subunit as an important M phase regulator and suggest that H4-K20 methylation status contributes to chromosome behavior during mitosis and proper cytokinesis.
Mots-clé
Cell Division/physiology, Cell Line, Tumor, Chromatin/metabolism, Cytokinesis, Hela Cells, Histone-Lysine N-Methyltransferase/metabolism, Histones/chemistry, Histones/metabolism, Host Cell Factor C1, Humans, Lysine/metabolism, Methylation, Mitosis/physiology, Protein Methyltransferases, RNA, Messenger/metabolism, RNA, Small Interfering/metabolism, Transcription Factors/genetics, Transcription Factors/metabolism, Up-Regulation
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 15:36
Dernière modification de la notice
20/08/2019 16:29
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