Wnt3a stimulation elicits G-protein-coupled receptor properties of mammalian Frizzled proteins.

Détails

Ressource 1Télécharger: BIB_FEA6F15E3924.P001.pdf (392.93 [Ko])
Etat: Public
Version: Final published version
ID Serval
serval:BIB_FEA6F15E3924
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Wnt3a stimulation elicits G-protein-coupled receptor properties of mammalian Frizzled proteins.
Périodique
Biochemical Journal
Auteur(s)
Koval A., Katanaev V.L.
ISSN
1470-8728 (Electronic)
ISSN-L
0264-6021
Statut éditorial
Publié
Date de publication
2011
Volume
433
Numéro
3
Pages
435-440
Langue
anglais
Résumé
Receptors of the Fz (Frizzled) family initiate Wnt ligand-dependent signalling controlling multiple steps in organism development and carcinogenesis. Fz proteins possess seven transmembrane domains, and their signalling depends on heterotrimeric G-proteins in various organisms; however, Fz proteins constitute a distinct group within the GPCR (G-protein-coupled receptor) superfamily, and Fz signalling can be G-protein-independent in some experimental setups, leading to concerns about the GPCR nature of these proteins. In the present study, we demonstrate that mammalian Fz proteins act as GPCRs on heterotrimeric G(o/i) proteins. Addition of the Wnt3a ligand to rat brain membranes or cultured cells elicits Fz-dependent guanine-nucleotide exchange on G(o/i) proteins. These responses were sensitive to a Wnt antagonist and to pertussis toxin, which decouples the G(o/i) proteins from their receptors through covalent modification. The results of the present study provide the long-awaited biochemical proof of the GPCR nature of Fz receptors.
Mots-clé
Animals, Brain, Cells, Cultured, Frizzled Receptors/metabolism, GTP-Binding Protein alpha Subunits, Gi-Go, Guanine Nucleotides/metabolism, Rats, Receptors, G-Protein-Coupled, Wnt Proteins/metabolism, Wnt3 Protein
Pubmed
Web of science
Création de la notice
21/12/2011 10:27
Dernière modification de la notice
20/08/2019 16:29
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