Detection of Epstein-Barr virus DNA by polymerase chain reaction in lymph node biopsies from patients with angioimmunoblastic lymphadenopathy

Détails

ID Serval
serval:BIB_FE62AE108550
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Detection of Epstein-Barr virus DNA by polymerase chain reaction in lymph node biopsies from patients with angioimmunoblastic lymphadenopathy
Périodique
British Journal of Haematology
Auteur(s)
Knecht  H., Sahli  R., Shaw  P., Meyer  C., Bachmann  E., Odermatt  B. F., Bachmann  F.
ISSN
0007-1048 (Print)
Statut éditorial
Publié
Date de publication
08/1990
Volume
75
Numéro
4
Pages
610-4
Notes
Journal Article --- Old month value: Aug
Résumé
Epstein-Barr virus DNA was detected by the polymerase chain reaction (PCR) in five lymph node biopsies from eight patients with diagnosis of angioimmunoblastic lymphadenopathy (AILD). Three pairs of specific primers detected EBV DNA sequences near the 5' end (Bam W region), the middle (BMRF 1 region) and the 3' end (Eco RI D region) of the viral genome with equal accuracy when 1 microgram of DNA and 30 amplification cycles were used. When only 100 ng of DNA were screened with the BMRF 1 set of primers, a specific amplification product was still identifiable. The 593 base pair amplification product obtained using the Eco RI D set of primers was shown to contain an expected Sma I site at position 215, confirming the viral origin of the amplified sequence [corrected]. Our findings indicate that lymph nodes of AILD patients frequently harbour the entire EBV genome at a high percentage of at least 1 viral copy per 15,000 human cells.
Mots-clé
Base Sequence DNA, Viral/*analysis Genes, Viral Herpesvirus 4, Human/*genetics Humans Immunoblastic Lymphadenopathy/*genetics/microbiology Lymph Nodes/microbiology Molecular Sequence Data Polymerase Chain Reaction
Pubmed
Web of science
Création de la notice
24/01/2008 21:51
Dernière modification de la notice
20/08/2019 17:29
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