Activation of p38 mitogen-activated protein kinase in spinal microglia contributes to incision-induced mechanical allodynia.

Détails

Ressource 1Télécharger: BIB_FE0356C8CF43.P001.pdf (541.10 [Ko])
Etat: Public
Version: Author's accepted manuscript
ID Serval
serval:BIB_FE0356C8CF43
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Activation of p38 mitogen-activated protein kinase in spinal microglia contributes to incision-induced mechanical allodynia.
Périodique
Anesthesiology
Auteur(s)
Wen Y.R., Suter M.R., Ji R.R., Yeh G.C., Wu Y.S., Wang K.C., Kohno T., Sun W.Z., Wang C.C.
ISSN
1528-1175 (Electronic)
ISSN-L
0003-3022
Statut éditorial
Publié
Date de publication
2009
Peer-reviewed
Oui
Volume
110
Numéro
1
Pages
155-165
Langue
anglais
Notes
Publication types: Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't Publication Status: ppublish
Résumé
BACKGROUND: Recent studies have implicated the activation of stress-activated mitogen-activated protein kinase (MAPK) p38 in spinal microglial cells for development of neuropathic and inflammatory pain. The aim of the present study was to investigate whether phosphorylation of p38 (p-p38) also mediates mechanical allodynia and thermal hyperalgesia induced by plantar incision.
METHODS: After rats received a plantar incision surgery, mechanical allodynia and thermal hyperalgesia were determined by von Frey filaments and radiant heat, respectively, and the number of p-p38 immunoreactive cells in the dorsal horn was quantified to determine p38 activation at different time points after incision. The p38 inhibitor FR167653 was administered intrathecally 30 min before hind paw plantar incision to determine the role of p38 in postoperative pain.
RESULTS: A significant increase in number of p-p38 immunoreactive cells was observed in the ipsilateral L4-5 spinal dorsal horn from 1 h to 3 days after the incision. p-p38 was found predominantly in microglia. However, microglial activation (assessed by OX-42 upregulation) was not evident until 3 days after plantar incision. Intrathecal pretreatment of FR167653 attenuated incision-induced mechanical allodynia from 1 h to day 2 and significantly reduced activation of p38 in the dorsal horn 1 day after plantar incision. However, FR167653 only inhibited heat hyperalgesia at an early time point.
CONCLUSIONS: Plantar incision-induced mechanical allodynia can be prevented by the p38 inhibitor. Our results suggest that p38 activation in spinal microglia play a role in incision-induced mechanical allodynia in rats. Therefore, p38 inhibition may be useful in treating postsurgical pain.
Mots-clé
Animals, Enzyme Activation/drug effects, Enzyme Activation/physiology, Male, Microglia/drug effects, Microglia/enzymology, Pain/enzymology, Pain/prevention & control, Pain Measurement/drug effects, Pain Measurement/instrumentation, Physical Stimulation/methods, Pyrazoles/pharmacology, Pyrazoles/therapeutic use, Pyridines/pharmacology, Pyridines/therapeutic use, Rats, Rats, Sprague-Dawley, Spinal Cord/drug effects, Spinal Cord/enzymology, p38 Mitogen-Activated Protein Kinases/antagonists & inhibitors, p38 Mitogen-Activated Protein Kinases/metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
08/01/2014 12:26
Dernière modification de la notice
20/08/2019 17:28
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