Wound-healing properties of trehalose-stabilized freeze-dried outdated platelets.

Détails

ID Serval
serval:BIB_FD7C2D39482B
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Wound-healing properties of trehalose-stabilized freeze-dried outdated platelets.
Périodique
Transfusion
Auteur(s)
Sum R., Hager S., Pietramaggiori G., Orgill D.P., Dee J., Rudolph A., Orser C., Fitzpatrick G.M., Ho D.
ISSN
0041-1132 (Print)
ISSN-L
0041-1132
Statut éditorial
Publié
Date de publication
04/2007
Peer-reviewed
Oui
Volume
47
Numéro
4
Pages
672-679
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, U.S. Gov't, Non-P.H.S.
Publication Status: ppublish
Résumé
The wound-healing applications of platelet (PLT)-derived cytokines, proteins, and membranes is accepted but continues to be investigated. In this study, it is demonstrated that stabilized freeze-dried PLTs prepared from outdated PLTs (FDPOs) accelerate wound healing and form tube structures as well as stabilized indated freeze-dried PLTs (FDPIs) and room-temperature fresh PLTs (RT-PLTs).
Experiments were designed to compare in vitro and in vivo wound-healing properties of FDPI, FDPO, and RT-PLT preparations. The concentration of PLT-derived growth factor (PDGF)-betabeta and transforming growth factor (TGF)-beta1 was determined, and the abilities of FDPIs, FDPOs and RT-PLTs to induce endothelial cell proliferation and promote endothelial cell tube formation (cells formed solid spouts connecting neighboring cells to form tube structures) were observed. Wound-healing characteristics were measured by surgically inducing 1-cm(2), full-thickness wounds on db/db mice (n = 10 per group). The wounds were treated with single or multiple doses of FDPIs and FDPOs. Wound closure rate was determined, and histology samples were evaluated for cellular makeup.
FDPOs retained the same levels of PDGF-betabeta and TGF-beta1 and were able to promote endothelial cell proliferation and tube formation in vitro as well as FDPIs or RT-PLTs. Multiple applications of FDPO accelerated wound closure and enhanced reepithelialization when compared to untreated wounds in db/db mice.
FDPOs enhanced wound healing in db/db mice as well as FDPIs and RT-PLTs. Wound closure was obtained 6 days earlier than untreated wounds and histologic examination revealed reduced granulation and increased cellular angiogenesis.

Mots-clé
Animals, Blood Platelets/drug effects, Blood Platelets/metabolism, Blood Platelets/physiology, Blood Preservation/methods, Cell Proliferation, Cells, Cultured, Diabetes Mellitus, Type 2/blood, Diabetes Mellitus, Type 2/physiopathology, Freeze Drying/methods, Humans, Male, Mice, Platelet Transfusion/methods, Platelet-Derived Growth Factor/metabolism, Transforming Growth Factor beta1/metabolism, Trehalose/pharmacology, Wound Healing
Pubmed
Web of science
Création de la notice
16/01/2018 15:44
Dernière modification de la notice
20/08/2019 17:28
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