Glucocorticoids and 11 beta-hydroxysteroid dehydrogenase type 2 gene expression in the aging kidney

Détails

ID Serval
serval:BIB_FD4FABC849AA
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Glucocorticoids and 11 beta-hydroxysteroid dehydrogenase type 2 gene expression in the aging kidney
Périodique
European Journal of Clinical Investigation
Auteur(s)
Audige  A., Dick  B., Frey  B. M., Frey  F. J., Corman  B., Vogt  B.
ISSN
0014-2972 (Print)
Statut éditorial
Publié
Date de publication
06/2002
Volume
32
Numéro
6
Pages
411-20
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jun
Résumé
BACKGROUND: Aging is associated with increased concentrations of circulating glucocorticoids, a situation expected to induce a glucocorticoid-mediated mineralocorticoid effect, resulting in sodium retention and hypertension unless counteracting mechanisms are operative. Conversion of glucocorticoids to inert 11 beta-keto compounds by the enzyme 11 beta-hydroxysteroid dehydrogenase type 2 (11 beta-HSD2) is one of these mechanisms. We hypothesized therefore that 11 beta-HSD2 gene expression and/or activity increase with age in male WAG/Rij rats, a strain without increased blood pressure with age or senescence-related obesity or kidney disease. MATERIALS AND METHODS: Corticosterone (B) concentrations in plasma and urinary excretion of corticosterone and dehydrocorticosterone (A) tetrahydro metabolites, THB + 5 alpha-THB + THA, were assessed by gas chromatography-mass spectrometry (GC-MS) in 10-month-old-rats (n = 6) and in 30-month-old rats (n = 6). Renal 11 beta-HSD2 messenger ribonucleic acid (mRNA) abundance was measured by real-time quantitative TaqMan polymerase chain reaction and microarray assays. RESULTS: Thirty-month-old rats had significantly higher corticosterone concentrations in plasma and increased urinary excretion of corticosterone and dehydrocorticosterone tetrahydro metabolites. Conversion of B to A in kidney microsomes from 30-month-old rats was moderately but not significantly increased compared with 10-month-old rats. The urinary ratios of (THB + 5 alpha-THB)/THA and free B/A and renal 11 beta-HSD2 mRNA abundance were equal in 10- and 30-month-old rats. CONCLUSIONS: There is no evidence for an enhanced gene expression or activity of renal 11 beta-HSD2 in these aging rats, suggesting either that endogenous 11 beta-HSD2 is able to cope with the increased corticosterone concentrations characteristic of the aging process or that alternative mechanisms contribute to the maintenance of a normal sodium excretion in these animals.
Mots-clé
11-beta-Hydroxysteroid Dehydrogenase Type 2 Aging/*physiology Animals Electrolytes/urine Gas Chromatography-Mass Spectrometry Gene Expression Regulation Glucocorticoids/*metabolism Humans Hydroxysteroid Dehydrogenases/*genetics Kidney/*enzymology Male Microsomes/metabolism Oligonucleotide Array Sequence Analysis Rats Rats, Inbred Strains
Pubmed
Web of science
Création de la notice
25/01/2008 13:03
Dernière modification de la notice
20/08/2019 16:28
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