Induction of sustained and elevated immune responses to weakly immunogenic synthetic malarial peptides by encapsulation in biodegradable polymer microspheres

Détails

ID Serval
serval:BIB_FD16B9FF12F1
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Induction of sustained and elevated immune responses to weakly immunogenic synthetic malarial peptides by encapsulation in biodegradable polymer microspheres
Périodique
Vaccine
Auteur(s)
Men  Y., Gander  B., Merkle  H. P., Corradin  G.
ISSN
0264-410X (Print)
Statut éditorial
Publié
Date de publication
10/1996
Volume
14
Numéro
15
Pages
1442-50
Notes
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Oct
Résumé
Biodegradable microspheres (MS) based on poly(D,L-lactide) and poly(D,L-lactide-coglycolide) have the capacity to release encapsulated antigens over defined lengths of time depending on their composition and to elicit and sustain strong and long-lasting immune responses to protein antigens. In the present study, two synthetic multiple antigenic peptides (MAP), P30B2 and (NANP)6P2P30, were incorporated into MS of different compositions. P30B2 and (NANP)6P2P30 are composed of one or two universal T helper epitopes from tetanus toxin, 947-967 (P30) and 830-843 (P2), and of a B cell epitope derived from the repeat sequence of Plasmodium berghei or Plasmodium falciparum, respectively. BALB/c mice were immunized with these two peptides in different formulations, including individual MS or mixtures of MS with various release properties, Incomplete Freund's adjuvant (IFA) or as soluble peptides. MS formulations elicited strong and sustained proliferative and antibody responses comparable to those obtained with the IFA preparations. Furthermore, MS formulations induced specific isotype/ subclass antibodies similar to those induced by IFA. No significant augmentation of total serum IgE was detected during this study. In addition, a boosting effect was obtained when the immunized mice were reinjected with a small antigen dose in IFA several months later. These results indicate that biodegradable MS may be a suitable vaccine delivery system/adjuvant not only for protein antigens but also for weakly immunogenic synthetic peptides.
Mots-clé
Amino Acid Sequence Animals Biocompatible Materials Drug Carriers *Drug Delivery Systems Epitopes/*administration & dosage/immunology Female Immunoglobulins/*blood *Lactic Acid Lymph Nodes/immunology Malaria Vaccines/*administration & dosage/*immunology Mice Mice, Inbred BALB C Microspheres Molecular Sequence Data Peptide Fragments/immunology Plasmodium berghei/*immunology Plasmodium falciparum/*immunology Polyesters *Polyglycolic Acid Polymers T-Lymphocytes/immunology Tetanus Toxin/immunology Tetanus Toxoid/immunology Time Factors Vaccines, Synthetic/administration & dosage
Pubmed
Web of science
Création de la notice
24/01/2008 14:55
Dernière modification de la notice
20/08/2019 16:28
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