Flow rate dependent ex vivo deheparinization with immobilized cationic ligand

Détails

ID Serval
serval:BIB_FCF4DF8EEC68
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Flow rate dependent ex vivo deheparinization with immobilized cationic ligand
Périodique
ASAIO Journal
Auteur(s)
Tevaearai  H. T., Mueller  X. M., Marty  B., Horisberger  J., Schapira  M., von Segesser  L. K.
ISSN
1058-2916 (Print)
Statut éditorial
Publié
Date de publication
10/1997
Volume
43
Numéro
5
Pages
M487-9
Notes
In Vitro
Journal Article --- Old month value: Sep-Oct
Résumé
The Heparin Removal Device is a deheparinization system that provides a good alternative when adverse reaction to protamine is suspected or when a heparin coated device is employed for cardiopulmonary bypass. In a bovine model, after systemic heparinization with 300 IU/kg body wt, the authors investigated the rate of deheparinization using this plasmapheresis system that allows exposition of heparinized plasma to an immobilized cationic ligand. Blood was shunted through a heparin coated veno-venous circuit to the plasma separator at a flow rate of 500 ml/min (n = 3, body wt 68 +/- 4 kg) or 1,000 ml/min (n = 3, body wt 57 +/- 4 kg). All plasma separators remained patent without any failure of the devices. Blood samples were drawn at regular intervals for coagulation parameters. The evolution of the activated coagulation time (ACT) was measured during the first 60 min of the deheparinization procedure, and results were compared with spontaneous evolution in a control group (n = 3, body wt 73 +/- 5 kg) without reversal of heparinization. After heparin administration, mean ACT was longer than 1,000 sec in the three groups. Fifteen minutes later it was still > 1,000 sec in the control group, whereas it was 427 +/- 8 sec in the slow flow group (500 ml/min) and 340 +/- 26 sec in the high flow group (1,000 ml/min). At 30 min it was 1,000, 367 +/- 26, and 200 +/- 24 sec, respectively, in the three groups. A regression curve was calculated for each group, and areas under the curves were compared. Ex vivo deheparinization was efficient in normalization of ACT, but removal of heparin appeared to be flow rate dependent. Increased blood flow from 500 to 1,000 ml/min across the deheparinization system resulted in significant acceleration of ACT normalization (p < 0.05).
Mots-clé
Animals Blood Flow Velocity Cardiopulmonary Bypass Cations Cattle Evaluation Studies Heparin/*blood/*isolation & purification Heparin Antagonists/adverse effects Ligands Plasmapheresis/*instrumentation/*methods Protamines/adverse effects Whole Blood Coagulation Time
Pubmed
Web of science
Création de la notice
25/01/2008 15:28
Dernière modification de la notice
20/08/2019 16:27
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