Observation of living cells using the atomic force microscope

Détails

ID Serval
serval:BIB_FCF17AE2205A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Observation of living cells using the atomic force microscope
Périodique
Biophysical Journal
Auteur(s)
Kasas  S., Gotzos  V., Celio  M. R.
ISSN
0006-3495
Statut éditorial
Publié
Date de publication
02/1993
Peer-reviewed
Oui
Volume
64
Numéro
2
Pages
539-544
Notes
Journal Article Research Support, Non-U.S. Gov't --- Old month value: Feb
Résumé
We used an atomic force microscope (AFM) to image samples immersed in a fluid in order to study the dynamic behavior of the membranes of living cells. AFM images of cultured cells immersed in a buffer were obtained without any preliminary preparation. We observed surface changes and displacements which suggest that the cells were still alive during the measurements. Some membrane details imaged with the AFM have also been observed using a scanning electron microscope and their dynamic behavior has been confirmed by microcinematography. We believe that the AFM will offer new insights into the exploration of dynamic changes affecting cell membranes.
Mots-clé
Biophysics Cell Line Cells/*ultrastructure Cytological Techniques Evaluation Studies Humans Microscopy/instrumentation/*methods Microscopy, Electron, Scanning
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 14:24
Dernière modification de la notice
20/08/2019 16:27
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