Increased fiber outgrowth from xeno-transplanted human embryonic dopaminergic neurons with co-implants of polymer-encapsulated genetically modified cells releasing glial cell line-derived neurotrophic factor

Détails

ID Serval
serval:BIB_FC68175623D1
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Increased fiber outgrowth from xeno-transplanted human embryonic dopaminergic neurons with co-implants of polymer-encapsulated genetically modified cells releasing glial cell line-derived neurotrophic factor
Périodique
Brain Research Bulletin
Auteur(s)
Ahn  Y. H., Bensadoun  J. C., Aebischer  P., Zurn  A. D., Seiger  A., Bjorklund  A., Lindvall  O., Wahlberg  L., Brundin  P., Kaminski Schierle  G. S.
ISSN
0361-9230 (Print)
Statut éditorial
Publié
Date de publication
07/2005
Volume
66
Numéro
2
Pages
135-42
Notes
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jul 30
Résumé
We investigated whether a continuous supply of glial cell line-derived neurotrophic factor (GDNF) via encapsulated genetically modified cells can promote survival and fiber outgrowth from xenotransplanted human dopaminergic neurons. Cells genetically engineered to continuously secrete GDNF were confined in hollow fiber-based macrocapsules. Each hemiparkinsonian rat received either a single C2C12-hGDNF capsule (n=8) or a C2C12-control capsule (n=8) concomitantly with human embryonic ventral mesencephalic cell suspension transplants. Our results show that fiber outgrowth in the area between the capsule and the graft is more extensive in rats with GDNF-releasing capsules than in rats with control capsules. We suggest that continuous and safe delivery of GDNF to the brain could be a potential way to optimize neural transplantation as a therapy for Parkinson's disease.
Mots-clé
Analysis of Variance Animals Behavior, Animal/drug effects/physiology Capsules Cell Size/drug effects Dopamine/*metabolism Embryo Female Fetal Tissue Transplantation/*methods Functional Laterality Genetic Engineering/methods Glial Cell Line-Derived Neurotrophic Factor Humans Immunohistochemistry/methods Implants, Experimental Mesencephalon/cytology Motor Activity/drug effects Nerve Fibers/*drug effects/physiology Nerve Growth Factors/biosynthesis/*pharmacology Neuroglia/physiology Neurons/*physiology Oxidopamine/toxicity Rats Rats, Sprague-Dawley Rotarod Performance Test/methods Time Factors Tyrosine 3-Monooxygenase/metabolism
Pubmed
Web of science
Création de la notice
28/01/2008 9:44
Dernière modification de la notice
20/08/2019 17:27
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