cis- and trans-acting elements of the estrogen-regulated vitellogenin gene B1 of Xenopus laevis.

Détails

ID Serval
serval:BIB_FC1DBE00DAF6
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
cis- and trans-acting elements of the estrogen-regulated vitellogenin gene B1 of Xenopus laevis.
Périodique
Journal of Steroid Biochemistry
Auteur(s)
Wahli W., Martinez E., Corthésy B., Cardinaux J.R.
ISSN
0960-0760
0022-4731[print], 0022-4731[linking]
Statut éditorial
Publié
Date de publication
1989
Peer-reviewed
Oui
Volume
34
Numéro
1-6
Pages
17-32
Langue
anglais
Notes
Publication types: Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Vitellogenin genes are expressed under strict estrogen control in the liver of female oviparous vertebrates. Gene transfer experiments using estrogen-responsive cells have shown that the 13 bp perfect palindromic element GGTCACTGTGACC found upstream of the Xenopus laevis vitellogenin gene A2 promoter mediates hormonal stimulation and thus, was called the estrogen-responsive element (ERE). In the Xenopus vitellogenin genes B1 and B2 there are two closely adjacent EREs with one or more base substitutions when compared to the consensus ERE GGTCANNNTGACC. On their own, these degenerated elements have only a low or no regulatory capacity at all but act together synergistically to form an estrogen-responsive unit (ERU) with the same strength as the perfect palindromic 13 bp element. Analysis of estrogen receptor binding to the gene B1 ERU revealed a cooperative interaction of receptor dimers to the two adjacent imperfect EREs which most likely explains the synergistic stimulation observed in vivo. Furthermore, a promoter activator element located between positions --113 and --42 of the gene B1 and functional in the human MCF-7 and the Xenopus B3.2 cells has been identified and shown to be involved in the high level of induced transcription activity when the ERE is placed at a distance from the promoter. Finally, a hormone-controlled in vitro transcription system derived from Xenopus liver nuclear extracts was exploited to characterize two additional novel cis-acting elements within the vitellogenin gene B1 promoter. One of them, a negative regulatory element (NRE), is responsible for repression of promoter activity in the absence of hormone. The second is related to the NF-I binding site and is required, together with the ERE, to mediate hormonal induction. Moreover, we detected three trans-acting activities in Xenopus liver nuclear extracts that interact with these regions and demonstrated that they participate in the regulation of the expression of the vitellogenin promoter in vitro.
Mots-clé
Animals, Base Sequence, Cell Line, Estrogens/pharmacology, Estrogens/physiology, Female, Genes/drug effects, Humans, Liver/metabolism, Male, Molecular Sequence Data, Multigene Family, Promoter Regions, Genetic/drug effects, Transcription, Genetic, Transcriptional Activation, Vitellogenins/genetics, Xenopus laevis
Pubmed
Web of science
Création de la notice
18/02/2008 11:32
Dernière modification de la notice
20/08/2019 17:27
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