Role of protein kinase activation in the induction of B cell adhesion by MHC class II ligands

Détails

ID Serval
serval:BIB_FBE4CD26D717
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Role of protein kinase activation in the induction of B cell adhesion by MHC class II ligands
Périodique
Journal of Immunology
Auteur(s)
Fuleihan  R., Spertini  F., Geha  R. S., Chatila  T.
ISSN
0022-1767 (Print)
Statut éditorial
Publié
Date de publication
09/1992
Volume
149
Numéro
6
Pages
1853-8
Notes
In Vitro
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Sep 15
Résumé
Engagement of MHC class II (Ia) molecules on B cells induces tyrosine phosphorylation, phosphoinositide turnover, elevation of intracellular calcium concentrations, and a rise in cAMP levels. However, a role for these biochemical signals in mediating functional responses induced by Ia ligands remains largely undefined. In this study, we utilized the induction of B cell adhesion by Ia ligands to demonstrate a role for signals transduced via Ia molecules in the generation of a functional response. Ia ligands that induced B cell aggregation induced tyrosine phosphorylation, whereas Ia ligands that did not induce B cell aggregation failed to induce any detectable tyrosine phosphorylation. Ia-induced B cell aggregation and tyrosine phosphorylation were inhibited by genistein and by herbimycin A, inhibitors of tyrosine kinases (PTK). Sphingosine and calphostin C, inhibitors of protein kinase C (PKC), also inhibited Ia-induced adhesion whereas HA1004, an inhibitor of cyclic nucleotide-dependent kinases, did not. Ia ligands induced both LFA-1-dependent and LFA-1-independent B cell adhesion. These two pathways of cell adhesion differed in their requirement for activation signals. PKC activation was sufficient for LFA-1-dependent adhesion, whereas LFA-1-independent adhesion required independent phosphorylation events mediated by PKC and by PTK. These results provide functional relevance for biochemical signals transduced via Ia molecules by demonstrating that Ia-induced B cell adhesion is mediated by the activation of PKC and by one or more PTK.
Mots-clé
B-Lymphocytes/*physiology Benzoquinones Cell Adhesion/*drug effects Enzyme Activation Genistein HLA-D Antigens/*physiology Humans Isoflavones/pharmacology Lactams, Macrocyclic Lymphocyte Function-Associated Antigen-1/metabolism Protein Kinase C/antagonists & inhibitors/*metabolism Protein-Tyrosine Kinases/antagonists & inhibitors/*metabolism Quinones/pharmacology Signal Transduction Sphingosine/pharmacology
Pubmed
Web of science
Création de la notice
25/01/2008 15:20
Dernière modification de la notice
20/08/2019 16:27
Données d'usage