Membrane vesicle secretion and prophage induction in multidrug-resistant Stenotrophomonas maltophilia in response to ciprofloxacin stress.

Détails

ID Serval
serval:BIB_FBCEC66653F9
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Membrane vesicle secretion and prophage induction in multidrug-resistant Stenotrophomonas maltophilia in response to ciprofloxacin stress.
Périodique
Environmental microbiology
Auteur⸱e⸱s
Devos S., Van Putte W., Vitse J., Van Driessche G., Stremersch S., Van Den Broek W., Raemdonck K., Braeckmans K., Stahlberg H., Kudryashev M., Savvides S.N., Devreese B.
ISSN
1462-2920 (Electronic)
ISSN-L
1462-2912
Statut éditorial
Publié
Date de publication
10/2017
Peer-reviewed
Oui
Volume
19
Numéro
10
Pages
3930-3937
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Several bacterial species produce membrane vesicles (MVs) in response to antibiotic stress. However, the biogenesis and role of MVs in bacterial antibiotic resistance mechanisms have remained unclear. Here, we studied the effect of the fluoroquinolone ciprofloxacin on MV secretion by Stenotrophomonas maltophilia using a combination of electron microscopy and proteomic approaches. We found that in addition to the classical outer membrane vesicles (OMV), ciprofloxacin-stimulated cultures produced larger vesicles containing both outer and inner membranes termed outer-inner membrane vesicles (OIMV), and that such MVs are enriched with cytosolic proteins. Remarkably, OIMV were found to be decorated with filamentous structures identified as fimbriae. In addition, ciprofloxacin stress leads to the release of bacteriophages and phage tail-like particles. Prophage induction by ciprofloxacin has been linked to pathogenesis and horizontal gene transfer in several bacterial species. Together, our findings show that ciprofloxacin treatment of S. maltophilia leads to the secretion of a heterogeneous pool of MVs and the induction of prophages that are potentially involved in adverse side-effects during antibiotic treatment.
Mots-clé
Anti-Bacterial Agents/pharmacology, Ciprofloxacin/pharmacology, Drug Resistance, Bacterial/drug effects, Fluoroquinolones/metabolism, Microbial Sensitivity Tests, Prophages/genetics, Prophages/physiology, Proteomics, Secretory Vesicles/drug effects, Secretory Vesicles/ultrastructure, Stenotrophomonas maltophilia/drug effects, Stenotrophomonas maltophilia/ultrastructure, Stenotrophomonas maltophilia/virology, Virus Activation/drug effects
Pubmed
Web of science
Création de la notice
09/06/2023 15:02
Dernière modification de la notice
08/07/2023 5:50
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