Blockade of ion channel expression in Xenopus oocytes with complementary DNA probes to Na+ and K+ channel mRNAs
Détails
ID Serval
serval:BIB_FB9C9EE2DECD
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Blockade of ion channel expression in Xenopus oocytes with complementary DNA probes to Na+ and K+ channel mRNAs
Périodique
Neuron
ISSN
0896-6273 (Print)
Statut éditorial
Publié
Date de publication
12/1988
Volume
1
Numéro
10
Pages
963-71
Notes
Journal Article --- Old month value: Dec
Résumé
Ionic currents were recorded from Xenopus oocytes injected with RNA isolated from chick or mouse brain. Three currents were studied: a rapid tetrodotoxin-sensitive Na+ current (Ina), an early outward K+ current sensitive to 4-aminopyridine (IA), and an inward current activated by the excitatory amino acid receptor agonist kainate. Oligonucleotides (60-80 bases long) complementary to rat brain Na+ channel sequences were prehybridized to chick brain RNA. These DNA sequences, upon injection into oocytes, specifically inhibited expression of INa relative to IA and the kainate-induced current in a dose-dependent manner. By contrast, prehybridization of oligonucleotides complementary to sequences either from the Drosophila Shaker locus (which codes for an early K+ current in Drosophila muscle) or from a homologous clone from mouse brain did not block the expression of the early outward K+ current induced in the oocytes by mRNA from chick or mouse brain. This method provides a convenient means for testing the functional role of cloned DNA species.
Mots-clé
Animals
Base Sequence
Cell Membrane/metabolism/physiology
Cell Membrane Permeability/drug effects/physiology
DNA/genetics/pharmacology
*DNA Probes
Drosophila/genetics
Electric Conductivity
Female
Genes, Regulator/physiology
Kainic Acid/pharmacology
Molecular Sequence Data
Oocytes/drug effects/*physiology/ultrastructure
Potassium/metabolism
Potassium Channels/*drug effects/physiology
Protein Biosynthesis
RNA, Messenger/*genetics/pharmacology
Sodium/metabolism
Sodium Channels/*drug effects/physiology
Xenopus/*physiology
Pubmed
Web of science
Création de la notice
24/01/2008 14:37
Dernière modification de la notice
20/08/2019 16:26