Fast gas chromatography and negative-ion chemical ionization tandem mass spectrometry for forensic analysis of cannabinoids in whole blood.

Détails

ID Serval
serval:BIB_FB658CD635F7
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Fast gas chromatography and negative-ion chemical ionization tandem mass spectrometry for forensic analysis of cannabinoids in whole blood.
Périodique
Journal of Pharmaceutical and Biomedical Analysis
Auteur⸱e⸱s
Thomas A., Widmer C., Hopfgartner G., Staub C.
ISSN
0731-7085 (Print)
ISSN-L
0731-7085
Statut éditorial
Publié
Date de publication
2007
Peer-reviewed
Oui
Volume
45
Numéro
3
Pages
495-503
Langue
anglais
Notes
Publication types: Journal Article Publication Status: ppublish
Résumé
The present work describes a fast gas chromatography/negative-ion chemical ionization tandem mass spectrometric assay (Fast GC/NICI-MS/MS) for analysis of tetrahydrocannabinol (THC), 11-hydroxy-tetrahydrocannabinol (THC-OH) and 11-nor-9-carboxy-tetrahydrocannabinol (THC-COOH) in whole blood. The cannabinoids were extracted from 500 microL of whole blood by a simple liquid-liquid extraction (LLE) and then derivatized by using trifluoroacetic anhydride (TFAA) and hexafluoro-2-propanol (HFIP) as fluorinated agents. Mass spectrometric detection of the analytes was performed in the selected reaction-monitoring mode on a triple quadrupole instrument after negative-ion chemical ionization. The assay was found to be linear in the concentration range of 0.5-20 ng/mL for THC and THC-OH, and of 2.5-100 ng/mL for THC-COOH. Repeatability and intermediate precision were found less than 12% for all concentrations tested. Under standard chromatographic conditions, the run cycle time would have been 15 min. By using fast conditions of separation, the assay analysis time has been reduced to 5 min, without compromising the chromatographic resolution. Finally, a simple approach for estimating the uncertainty measurement is presented.
Mots-clé
Cannabinoids/blood, Cannabinoids/chemistry, Forensic Toxicology, Gas Chromatography-Mass Spectrometry/methods, Humans, Molecular Structure, Reference Standards, Reproducibility of Results, Sensitivity and Specificity, Substance Abuse Detection/methods, Tandem Mass Spectrometry
Pubmed
Web of science
Création de la notice
23/02/2015 15:23
Dernière modification de la notice
20/08/2019 16:26
Données d'usage