Nonionic diffusion of salicylic acid through MDCK cell monolayers

Détails

ID Serval
serval:BIB_FB1E8C4834ED
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Nonionic diffusion of salicylic acid through MDCK cell monolayers
Périodique
Journal of Pharmacology and Experimental Therapeutics
Auteur(s)
Chatton  J. Y., Roch-Ramel  F.
ISSN
0022-3565 (Print)
Statut éditorial
Publié
Date de publication
06/1992
Volume
261
Numéro
3
Pages
1071-9
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jun
Résumé
MDCK-I cells, grown on collagen-coated filters, were used as a model of the distal nephron to study the nonionic diffusion of salicylic acid. The apparent transcellular permeability (PSal, centimeters per second) and intracellular content (ContSal, picomoles) of [14C]salicylic acid (ionized and nonionized) were measured from both apical-to-basolateral and basolateral-to-apical unidirectional fluxes. During the measurement of apical-to-basolateral fluxes at 21 degrees C, both PSal and ContSal correlated directly with the proportion of nonionized salicylic acid in the apical medium over the pH range 6.0-7.4. This indicated that the observed permeability was due to pure nonionic diffusion. However, in the basolateral-to-apical direction, the correlation was less evident and ContSal was 10 to 20 times higher, although PSal was of the same magnitude. These observations could not be explained by facilitated transport of anionic salicylic acid, at 21 degrees C, because 1 mM probenecid, 1 mM p-aminohippurate and 1 mM cold salicylic acid did not abolish the higher intracellular content obtained during basolateral-to-apical fluxes. Raising the temperature produced only a gradual increase of PSal measured in the basolateral-to-apical direction and an unexpected decrease of ContSal. Because basolateral-to-apical facilitated transport does not play a significant role, the most plausible reason for the higher ContSal during basolateral-to-apical fluxes is that the apical membrane possesses a much lower (10- to 20-fold less) salicylic acid permeability than the basolateral membrane and constitutes the rate-limiting step for transcellular salicylic acid flux.
Mots-clé
Animals Cell Membrane Permeability/drug effects Cells, Cultured Diffusion Dogs Hydrogen-Ion Concentration Kidney Cortex/metabolism Probenecid/pharmacology Salicylic Acid Salicylic Acids/*pharmacokinetics
Pubmed
Web of science
Création de la notice
24/01/2008 13:08
Dernière modification de la notice
20/08/2019 16:26
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