Important role of the long terminal repeat of the helper Moloney murine leukemia virus in Abelson virus-induced lymphoma.

Détails

ID Serval
serval:BIB_FAF86B36BCF1
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Important role of the long terminal repeat of the helper Moloney murine leukemia virus in Abelson virus-induced lymphoma.
Périodique
Journal of Virology
Auteur⸱e⸱s
Savard P., DesGroseillers L., Rassart E., Poirier Y., Jolicoeur P.
ISSN
0022-538X (Print)
ISSN-L
0022-538X
Statut éditorial
Publié
Date de publication
1987
Volume
61
Numéro
10
Pages
3266-3275
Langue
anglais
Résumé
The helper virus has been shown to play a critical role in the development of lymphoma induced by the defective Abelson murine leukemia virus (A-MuLV). Indeed, A-MuLV pseudotyped with some viruses, such as the Moloney MuLV, has been shown to be highly lymphogenic, whereas A-MuLV pseudotyped with other viruses, such as the BALB/c endogenous N-tropic MuLV, has been shown to be devoid of lymphogenic potential (N. Rosenberg and D. Baltimore, J. Exp. Med. 147:1126-1141, 1978; C. D. Scher, J. Exp. Med. 147: 1044-1053, 1978). To map the viral DNA sequences encoding the determinant of the lymphogenic potential of Moloney MuLV when complexed with A-MuLV, we constructed chimeric helper viral DNA genomes in vitro between parental cloned infectious viral DNA genomes from Moloney MuLV and from BALB/c endogenous N-tropic MuLV. Chimeric helper MuLVs, recovered after transfection of NIH 3T3 cells were used to rescue A-MuLV, and the pseudotypes were inoculated into newborn NIH Swiss, CD-1, and SWR/J mice to test their lymphogenic potential. We found that a 0.44-kilobase-pair PstI-KpnI long terminal repeat-containing fragment from the Moloney MuLV was sufficient to confer some, but not complete, lymphogenic potential to a chimeric virus (p7M2) in NIH Swiss and SWR/J mice, but not in CD-1 mice. The addition of the 3'-end env sequences (comprising the carboxy terminus of gp70 and all p15E) to the U3 long terminal repeat sequences restored the full lymphogenic potential of the Moloney MuLV. Our data indicate that the 3'-end sequences of the helper Moloney MuLV are somehow involved in the development of lymphoma induced by A-MuLV. The same sequences have previously been found to harbor the determinant of leukemogenicity and of disease specificity of Moloney MuLV when inoculated alone.
Mots-clé
Abelson murine leukemia virus/genetics, Animals, Animals, Newborn, Base Sequence, Cell Line, Chimera, Cloning, Molecular, DNA Restriction Enzymes, DNA, Neoplasm/analysis, DNA, Viral/analysis, DNA, Viral/genetics, Genes, Viral, Helper Viruses/genetics, Leukemia Virus, Murine/genetics, Leukemia, Experimental/microbiology, Mice, Moloney murine leukemia virus/genetics, Nucleic Acid Hybridization, Repetitive Sequences, Nucleic Acid, Transfection
Pubmed
Web of science
Création de la notice
26/02/2008 12:05
Dernière modification de la notice
20/08/2019 16:26
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