Regulation of energy metabolism by neurotransmitters in astrocytes in primary culture and in an immortalized cell line

Détails

ID Serval
serval:BIB_F90E9868CF48
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Regulation of energy metabolism by neurotransmitters in astrocytes in primary culture and in an immortalized cell line
Périodique
Glia
Auteur(s)
Pellerin  L., Stolz  M., Sorg  O., Martin  J. L., Deschepper  C. F., Magistretti  P. J.
ISSN
0894-1491
Statut éditorial
Publié
Date de publication
09/1997
Peer-reviewed
Oui
Volume
21
Numéro
1
Pages
74-83
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Sep
Résumé
Evidence suggests that astrocytes might play an important role in cerebral energy metabolism. A recently developed cell line, called DI TNC1, displays several characteristic features of astrocytes. Thus, we have investigated in these cells a number of parameters related to energy metabolism. First, glycogen, the major energy reserve in the brain, is present in these cells and its levels are influenced by the glucose content of the growth medium and the presence of serum. Second, several neurotransmitters including noradrenaline and vasoactive intestinal peptide (VIP) induce a glycogenolytic response. Their effect on glycogen is paralleled by a similar effect on the formation of cyclic AMP, which is presumably the second messenger involved. Third, noradrenaline stimulates glucose utilization (as reflected by 2-deoxyglucose uptake) in DI TNC1 cells, an effect which is mimicked by the second messenger arachidonate. Interestingly, two actions of neurotransmitters, which are well characterized in primary astrocytes, are absent in DI TNC1 cells. These are the noradrenaline- and VIP-induced resynthesis of glycogen and the glutamate-stimulated glycolysis. In summary, the observations reported here lend further support to the concept that astrocytes are important for the control of brain energy metabolism. In addition, DI TNC1 cells might represent an interesting preparation to help decipher some of the astrocytic functions related to energy metabolism.
Mots-clé
Animals Arachidonic Acid/metabolism Astrocytes/drug effects/*metabolism Brain/*metabolism Cell Line Cell Line, Transformed Deoxyglucose/metabolism Energy Metabolism/*drug effects Glucose/metabolism Glutamic Acid/pharmacology Glycogen/metabolism Glycolysis/drug effects Neurotransmitter Agents/*pharmacology Norepinephrine/pharmacology Rats Second Messenger Systems Vasoactive Intestinal Peptide/pharmacology
Pubmed
Web of science
Création de la notice
20/01/2008 19:22
Dernière modification de la notice
20/08/2019 17:24
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