Pob1 participates in the Cdc42 regulation of fission yeast actin cytoskeleton.
Détails
ID Serval
serval:BIB_F9055D3C4CB4
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Pob1 participates in the Cdc42 regulation of fission yeast actin cytoskeleton.
Périodique
Molecular Biology of the Cell
ISSN
1939-4586 (Electronic)
ISSN-L
1059-1524
Statut éditorial
Publié
Date de publication
2009
Volume
20
Numéro
20
Pages
4390-4399
Langue
anglais
Résumé
Rho GTPases regulate the actin cytoskeleton in all eukaryotes. Fission yeast Cdc42 is involved in actin cable assembly and formin For3 regulation. We isolated cdc42-879 as a thermosensitive strain with actin cable and For3 localization defects. In a multicopy suppressor screening, we identified pob1(+) as suppressor of cdc42-879 thermosensitivity. Pob1 overexpression also partially restores actin cables and localization of For3 in the mutant strain. Pob1 interacts with Cdc42 and this GTPase regulates Pob1 localization and/or stability. The C-terminal pleckstrin homology (PH) domain of Pob1 is required for Cdc42 binding. Pob1 also binds to For3 through its N-terminal sterile alpha motif (SAM) domain and contributes to the formin localization at the cell tips. The previously described pob1-664 mutant strain (Mol. Biol. Cell. 10, 2745-2757, 1999), which carries a mutation in the PH domain, as well as pob1 mutant strains in which Pob1 lacks the N-terminal region (pob1DeltaN) or the SAM domain (pob1DeltaSAM), have cytoskeletal defects similar to that of cdc42-879 cells. Expression of constitutively active For3DAD* partially restores actin organization in cdc42-879, pob1-664, pob1DeltaN, and pob1DeltaSAM. Therefore, we propose that Pob1 is required for For3 localization to the tips and facilitates Cdc42-mediated relief of For3 autoinhibition to stimulate actin cable formation.
Mots-clé
Actin Cytoskeleton/metabolism, Actin Cytoskeleton/ultrastructure, Actins/metabolism, Amino Acid Substitution, Cell Cycle Proteins/chemistry, Cell Cycle Proteins/physiology, Cell Polarity, Cytoskeleton/ultrastructure, Gene Expression Regulation, Fungal, Guanosine Triphosphate/physiology, Protein Interaction Mapping, Protein Structure, Tertiary, Schizosaccharomyces/metabolism, Schizosaccharomyces/ultrastructure, Schizosaccharomyces pombe Proteins/chemistry, Schizosaccharomyces pombe Proteins/genetics, cdc42 GTP-Binding Protein/chemistry, cdc42 GTP-Binding Protein/genetics, rho GTP-Binding Proteins/chemistry, rho GTP-Binding Proteins/physiology
Pubmed
Web of science
Création de la notice
03/11/2009 16:29
Dernière modification de la notice
20/08/2019 17:24