Immunomarquage des collagènes I, III et IV, de la laminine et de la fibronectine dans la capsule cristallinienne humaine [Immunolabelling of collagen types I, III and IV, laminin and fibronectin in the human lens capsule].

Détails

ID Serval
serval:BIB_F8FCDD90A08E
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Immunomarquage des collagènes I, III et IV, de la laminine et de la fibronectine dans la capsule cristallinienne humaine [Immunolabelling of collagen types I, III and IV, laminin and fibronectin in the human lens capsule].
Périodique
Journal Français D'ophtalmologie
Auteur⸱e⸱s
Majo F., Montard M., Delbosc B., Kantelip B.
ISSN
0181-5512 (Print)
ISSN-L
0181-5512
Statut éditorial
Publié
Date de publication
1997
Peer-reviewed
Oui
Volume
20
Numéro
9
Pages
664-670
Langue
français
Notes
Publication types: English Abstract ; Journal ArticlePublication Status: ppublish
Résumé
PURPOSE: To localize collagen types I, III, and IV, laminin and fibronectin in the anterior human lens capsule.
MATERIAL AND METHODS: Twenty-one anterior capsules were sampled by capsulorhexis during extracapsular cataract extraction (mean age 71.5). All capsules were labelled by an immunostaining specific for each antibodies. Immunostaining of four capsules was revealed with immunoperoxydase and seventeen using indirect immunofluorescence.
RESULTS: Labelling of collagen types I and III was observed throughout the entire thickness of the capsule for each technique, the strongest labelling was found in the base of the epithelial cells with immunofluorescence. Collagen type IV was observed at the base of the epithelial cells whichever technique was used. Laminin could be detected in the inner layer of the capsule, using immunoperoxydase or immunofluorescence. No specific labelling was found for fibronectin using the two techniques.
CONCLUSIONS: Different kinds of collagens have been found in capsules, more particularly the type III. The latter does not appear on other ocular basement membrane. Because of this uneven distribution in the capsule's thickness, each collagen might have a specific function.
Mots-clé
Aged, Aged, 80 and over, Collagen/analysis, Female, Fibronectins/analysis, Fluorescent Antibody Technique, Indirect, Humans, Immunoenzyme Techniques, Laminin/analysis, Lens Capsule, Crystalline/chemistry, Male, Middle Aged
Pubmed
Web of science
Création de la notice
07/01/2013 11:16
Dernière modification de la notice
20/08/2019 16:24
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